-transparent.png){kind=logo}
In the solanaceous plant Nicotiana alata, self-incompatibility is controlled by a single, multiallelic locus (S locus) expressed in both pollen and pistil. Previously, we have shown cosegregation between alleles of the S locus and alleles of a gene that encodes a glycoprotein with ribonuclease activity (S-RNase). Furthermore, expression of the S-RNase gene is apparently confined to the pistil and is correlated with the onset of self-incompatibility. In this paper, we report that the S-RNase gene is also expressed at low levels in developing pollen. A transcript in developing pollen hybridized to a cDNA encoding the S2-RNase allele of the parent plant and did not hybridize to cDNAs encoding other S-RNase alleles. Two cDNAs for the S2-RNase were cloned from a library derived from anthers of a plant homozygous for the S2 allele and both corresponded to the coding sequence of the S2-RNase. The product of the S-RNase gene was detected by immunocytochemistry in the intine of mature, hydrated pollen grains. These results are interpreted in the light of current knowledge of the structure of the S locus.
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
February 1994,
Nature,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
August 1995,
Plant molecular biology,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
March 1991,
The Plant cell,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
February 2006,
The Journal of biological chemistry,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
May 1996,
Plant molecular biology,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
November 2013,
Annals of botany,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
April 1997,
The Plant journal : for cell and molecular biology,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
August 1995,
Plant molecular biology,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
December 1998,
The Plant journal : for cell and molecular biology,
P N Dodds, and
I Bönig, and
H Du, and
J Rödin, and
M A Anderson, and
E Newbigin, and
A E Clarke
May 1989,
The Plant cell,
