Biomaterial Database

Ultrastructural observations of the incorporation of guinea-pig spermatozoa into zona-free hamster oocytes. 1977

C Barros, and E Herrera

Zona-free hamster oocytes inseminated in vitro with acrosome-reacted guinea-pig spermatozoa were examined with the electron microscope. Guinea-pig spermatozoa, in the vicinity of the oocytes, consistently lacked the whole acrosome including the equatorial segment region. In cross fertilization sperm-egg membrane fusion does not differ significantly from that of normal fertilization. However, it was sometimes possible to observe protrusions of oocyte cytoplasm containing sperm chromatin in the process of dispersion.

UI	MeSH Term	Description	Entries
D007315	Insemination, Artificial	Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.	Artificial Insemination,Eutelegenesis,Artificial Inseminations,Eutelegeneses,Inseminations, Artificial
D008297	Male		Males
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D009865	Oocytes	Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).	Ovocytes,Oocyte,Ovocyte
D010063	Ovum	A mature haploid female germ cell extruded from the OVARY at OVULATION.	Egg,Egg, Unfertilized,Ova,Eggs, Unfertilized,Unfertilized Egg,Unfertilized Eggs
D005260	Female		Females
D005306	Fertilization	The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.	Conception,Fertilization, Delayed,Fertilization, Polyspermic,Conceptions,Delayed Fertilization,Delayed Fertilizations,Fertilizations,Fertilizations, Delayed,Fertilizations, Polyspermic,Polyspermic Fertilization,Polyspermic Fertilizations
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D006824	Hybridization, Genetic	The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.	Crossbreeding,Hybridization, Intraspecies,Crossbreedings,Genetic Hybridization,Genetic Hybridizations,Hybridizations, Genetic,Hybridizations, Intraspecies,Intraspecies Hybridization,Intraspecies Hybridizations