BIOMAT Database Logo BIOMAT Database Logo

Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing. 1993

B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Antiviral Therapeutic Research Unit, Wellcome Foundation Limited, Beckenham, Kent, UK.

A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D003062 Codon A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE). Codon, Sense,Sense Codon,Codons,Codons, Sense,Sense Codons
D004259 DNA-Directed DNA Polymerase DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair. DNA Polymerase,DNA Polymerases,DNA-Dependent DNA Polymerases,DNA Polymerase N3,DNA Dependent DNA Polymerases,DNA Directed DNA Polymerase,DNA Polymerase, DNA-Directed,DNA Polymerases, DNA-Dependent,Polymerase N3, DNA,Polymerase, DNA,Polymerase, DNA-Directed DNA,Polymerases, DNA,Polymerases, DNA-Dependent DNA
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004352 Drug Resistance, Microbial The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Antibiotic Resistance,Antibiotic Resistance, Microbial,Antimicrobial Resistance, Drug,Antimicrobial Drug Resistance,Antimicrobial Drug Resistances,Antimicrobial Resistances, Drug,Drug Antimicrobial Resistance,Drug Antimicrobial Resistances,Drug Resistances, Microbial,Resistance, Antibiotic,Resistance, Drug Antimicrobial,Resistances, Drug Antimicrobial
D001331 Automation Controlled operation of an apparatus, process, or system by mechanical or electronic devices that take the place of human organs of observation, effort, and decision. (From Webster's Collegiate Dictionary, 1993) Automations
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012984 Software Sequential operating programs and data which instruct the functioning of a digital computer. Computer Programs,Computer Software,Open Source Software,Software Engineering,Software Tools,Computer Applications Software,Computer Programs and Programming,Computer Software Applications,Application, Computer Software,Applications Software, Computer,Applications Softwares, Computer,Applications, Computer Software,Computer Applications Softwares,Computer Program,Computer Software Application,Engineering, Software,Open Source Softwares,Program, Computer,Programs, Computer,Software Application, Computer,Software Applications, Computer,Software Tool,Software, Computer,Software, Computer Applications,Software, Open Source,Softwares, Computer Applications,Softwares, Open Source,Source Software, Open,Source Softwares, Open,Tool, Software,Tools, Software
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human

Related Publications

B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
HIV-1 drug resistance mutations detection and HIV-1 subtype G report by using next-generation sequencing platform.
September 2020, Microbial pathogenesis,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
HIV-1C proviral DNA for detection of drug resistance mutations.
January 2018, PloS one,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Detection of low-frequency HIV type 1 reverse transcriptase drug resistance mutations by ultradeep sequencing in naive HIV type 1-infected individuals.
February 2014, AIDS research and human retroviruses,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Routine drug resistance testing in HIV-1 proviral DNA, using an automated next- generation sequencing assay.
December 2019, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Detection of cytomegalovirus drug resistance mutations by next-generation sequencing.
November 2013, Journal of clinical microbiology,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Correction: HIV-1C proviral DNA for detection of drug resistance mutations.
January 2018, PloS one,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
HIV Drug Resistance Mutations Detection by Next-Generation Sequencing during Antiretroviral Therapy Interruption in China.
February 2021, Pathogens (Basel, Switzerland),
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Simple detection of point mutations associated with HIV-1 drug resistance.
April 2001, Journal of virological methods,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
[Detection of resistance mutations in proviral DNA in HIV-1 infection].
February 2013, Enfermedades infecciosas y microbiologia clinica,
B A Larder, and A Kohli, and P Kellam, and S D Kemp, and M Kronick, and R D Henfrey
Drug resistance mutations in HIV-1.
January 2003, Topics in HIV medicine : a publication of the International AIDS Society, USA,
Copied contents to your clipboard!