Biomaterial Database

Use of bacteriophage for the selective isolation of thermophilic actinomycetes from composted eucalyptus bark. 1993

D I Kurtböke, and N E Murphy, and K Sivasithamparam

University of Western Australia, School of Agriculture, Soil Science and Plant Nutrition, Nedlands, Perth.

A method was developed to reduce the numbers of thermophilic bacteria on isolation plates, which in turn facilitated the detection and isolation of thermophilic actinomycetes. The method involves exposing the test material to bacteriophage suspensions prior to inoculation on isolation plates. This method was applied to composted eucalyptus bark samples, which were then inoculated on R8 and 1/2 TSA + 0.2% casein hydrolysate agar plates. The phage susceptibility of thermophilic bacteria provided a selective means of reducing their numbers on isolation plates and hence increased the numbers of Thermomonospora, Saccharopolyspora rectivirgula, and thermophilic Streptomyces spp. on these media in comparison with the numbers recorded from control plates.

UI	MeSH Term	Description	Entries
D008848	Micromonosporaceae	A family of gram-positive, saprophytic bacteria occurring in soil and aquatic environments.
D009172	Mycology	The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
D010946	Plants, Medicinal	Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.	Herbs, Medicinal,Medicinal Herbs,Healing Plants,Medicinal Plants,Pharmaceutical Plants,Healing Plant,Herb, Medicinal,Medicinal Herb,Medicinal Plant,Pharmaceutical Plant,Plant, Healing,Plant, Medicinal,Plant, Pharmaceutical,Plants, Healing,Plants, Pharmaceutical
D011549	Pseudomonas	A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.	Chryseomonas,Pseudomona,Flavimonas
D005052	Eucalyptus	A genus of trees of the Myrtaceae family, native to Australia, that yields gums, oils, and resins which are used as flavoring agents, astringents, and aromatics.	Blue Gum Tree,Bluegum Eucalyptus,Eucalyptus globulus,Blue Gum Trees,Eucalyptus, Bluegum,Gum Tree, Blue,Gum Trees, Blue,Tree, Blue Gum,Trees, Blue Gum
D000191	Actinomycetaceae	A family of bacteria including numerous parasitic and pathogenic forms.
D000383	Agriculture	The science, art or practice of cultivating soil, producing crops, and raising livestock.	Agronomy,Agricultural Development,Farming,Agronomies,Development, Agricultural
D001407	Bacillus	A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.	Bacillus bacterium
D001435	Bacteriophages	Viruses whose hosts are bacterial cells.	Phages,Bacteriophage,Phage
D012988	Soil Microbiology	The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.	Microbiology, Soil