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[Use of electrical impulses for selective isolation of actinomycetes f rom soil]. 1998

T I Bulina, and L P Terekhova, and M V Tiurin
Research Institute of New Antibiotics, Russian Academy of Medical Sciences, Moscow, Russia.

A new method was elaborated for the selective isolation of actinomycetes from soil, which involves the pretreatment of soil samples with electric pulses. The treatment of soil with 3-ms electric pulses at a field intensity of 12 kV/cm2 resulted in a significant (threefold on average) increase in the proportion of representatives of rare actinomycetes isolated. This treatment regime was found to be most efficient for the selective isolation of representatives of the genera Actinoplanes, Micromonospora, Actinomadura, and Microtetraspora. The proportion of representatives of the genera Streptosporangium and Saccharotrix also increased. The treatment of soil samples with 3-ms electric pulses at a field intensity of 16 kV/cm resulted in highly selective isolation of representatives of the genus Nocardioides.

UI MeSH Term Description Entries
D008828 Microbiological Techniques Techniques used in microbiology. Microbiologic Technic,Microbiologic Technics,Microbiologic Technique,Microbiological Technics,Technic, Microbiologic,Technics, Microbiological,Technique, Microbiologic,Techniques, Microbiologic,Microbiologic Techniques,Microbiological Technic,Microbiological Technique,Technic, Microbiological,Technics, Microbiologic,Technique, Microbiological,Techniques, Microbiological
D004560 Electricity The physical effects involving the presence of electric charges at rest and in motion.
D000192 Actinomycetales An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments. Corynebacteriaceae,Coryneform Group
D012988 Soil Microbiology The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms. Microbiology, Soil

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