Biomaterial Database

Assessment of fetal lung maturity by fluorescence polarization in high-risk pregnancies. 1993

A T Ruch, and R R Lenke, and E R Ashwood

Center for Women and Children, Toledo Hospital/Medical College of Ohio.

Although the validity of amniotic fluid fluorescence polarization (FPOL) has been documented in normal pregnancies, data are lacking on the predictive value of this method in high-risk pregnancies where biochemical maturation of the fetal lung may be altered. In this study, amniotic fluid was obtained from 86 women with pregnancies complicated by insulin-dependent diabetes (42), twin gestation (22), Rh sensitization (13) and known fetal anomalies (9). In all groups, when FPOL was > .280 (immature), phosphatidylglycerol (PG) was always absent and lecithin and sphingomyelin ratio (L/S) was < 2.5:1. When FPOL values were < .260 (mature), PG was always present and L/S was > 2.5 in 45/50 samples. When FPOL values ranged between .260-.280 (intermediate), L/S and PG values varied and were inconsistent. We conclude that FPOL assessment of fetal lung maturity in pregnancies complicated by diabetes, Rh sensitization, twin gestation and fetal anomalies is as accurate a procedure as is fetal lung maturity testing by L/S and PG. In addition, the testing procedure is quicker, more reproducible and, possibly, more reliable.

UI	MeSH Term	Description	Entries
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D009327	4-Chloro-7-nitrobenzofurazan	A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.	Chloronitrobenzoxadiazole,NBD Chloride,7-Chloro-4-nitrobenzofurazan,NBF-Cl,Nitrobenzoxadiazole Chloride,4 Chloro 7 nitrobenzofurazan,7 Chloro 4 nitrobenzofurazan,Chloride, NBD,Chloride, Nitrobenzoxadiazole,NBF Cl
D010713	Phosphatidylcholines	Derivatives of PHOSPHATIDIC ACIDS in which the phosphoric acid is bound in ester linkage to a CHOLINE moiety.	Choline Phosphoglycerides,Choline Glycerophospholipids,Phosphatidyl Choline,Phosphatidyl Cholines,Phosphatidylcholine,Choline, Phosphatidyl,Cholines, Phosphatidyl,Glycerophospholipids, Choline,Phosphoglycerides, Choline
D010715	Phosphatidylglycerols	A nitrogen-free class of lipids present in animal and particularly plant tissues and composed of one mole of glycerol and 1 or 2 moles of phosphatidic acid. Members of this group differ from one another in the nature of the fatty acids released on hydrolysis.	Glycerol Phosphoglycerides,Monophosphatidylglycerols,Phosphatidylglycerol,Phosphatidyl Glycerol,Glycerol, Phosphatidyl,Phosphoglycerides, Glycerol
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D011248	Pregnancy Complications	Conditions or pathological processes associated with pregnancy. They can occur during or after pregnancy, and range from minor discomforts to serious diseases that require medical interventions. They include diseases in pregnant females, and pregnancies in females with diseases.	Adverse Birth Outcomes,Complications, Pregnancy,Adverse Birth Outcome,Birth Outcome, Adverse,Complication, Pregnancy,Outcome, Adverse Birth,Pregnancy Complication
D011446	Prospective Studies	Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.	Prospective Study,Studies, Prospective,Study, Prospective
D005260	Female		Females
D005325	Fetal Organ Maturity	Functional competence of specific organs or body systems of the FETUS in utero.	Fetal Maturity, Functional,Functional Fetal Maturity,Maturity, Fetal Organ,Maturity, Functional Fetal,Organ Maturity, Fetal
D005454	Fluorescence Polarization	Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.	Anisotropy, Fluorescence,Fluorescence Anisotropy,Polarization, Fluorescence,Anisotropies, Fluorescence,Fluorescence Anisotropies,Fluorescence Polarizations,Polarizations, Fluorescence