-transparent.png){kind=logo}
Recently, we reported that GM1492 human diploid skin fibroblasts derived from a Bloom's patient upon UV-C irradiation fail to increase p53 to a detectable level and nevertheless accumulate in the G1-phase of the cell-cycle. Here we show that in GM1492 cells other types of DNA-damaging agents also fail to induce p53 as well as WAF1, a p53-regulated gene product involved in G1 cell-cycle arrest. Furthermore, the p53-dependent G1 cell-cycle checkpoint is indeed defective in these cells: However, induction of GADD45 mRNA still occurs in GM1492 after irradiation with UV-C. Since GADD45 is known to inhibit the entry into S, these data suggest that the observed accumulation of GM1492 cells in G1 after UV-C irradiation occurs at the G1/S boundary and is due to an inhibition of initiation of DNA-replication.
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
October 1994,
Cancer research,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
January 1997,
The Journal of biological chemistry,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
January 1996,
Biochimica et biophysica acta,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
January 1994,
Mutation research,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
May 1986,
Cell biology international reports,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
March 1999,
Molecular and cellular biology,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
May 2000,
Cancer research,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
February 2000,
Radiation research,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
April 2024,
Genes,
T van Laar, and
W T Steegenga, and
A G Jochemsen, and
C Terleth, and
A J van der Eb
July 1997,
Oncogene,
