BIOMAT Database Logo BIOMAT Database Logo

Myocyte nuclear mitotic division and programmed myocyte cell death characterize the cardiac myopathy induced by rapid ventricular pacing in dogs. 1995

Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Department of Medicine, New York Medical College, Valhalla, USA.

BACKGROUND Observations in humans have raised the possibility that idiopathic dilated cardiomyopathy is characterized by myocyte cell loss and cell proliferation, which contribute to wall thinning and chamber dilation. Moreover, the mechanism of myocyte cell death in this patient population has been unclear. Because rapid ventricular pacing in dogs leads to a dilated myopathy that mimics the idiopathic form in man, this animal model was used to demonstrate whether myocyte nuclear mitotic division and programmed myocyte cell death occur in this setting. Additionally, the expression of proliferating cell nuclear antigen (PCNA) and Fas protein in myocytes was examined as a molecular indicator of the activation of the cell cycle and apoptotic cell death, respectively. METHODS Mongrel dogs were chronically instrumented for measurements of systemic hemodynamics and for left ventricular pacing. At sacrifice, myocardial samples were obtained for the estimation of the number of myocytes and interstitial cells showing mitosis and for the detection of DNA laddering. In addition, the number of myocyte nuclei exhibiting DNA strand breaks, as well as the frequency of myocytes labeled by PCNA and Fas protein, was determined. Finally, the distribution of nuclei in enzymatically dissociated myocytes was evaluated. RESULTS Pacing-induced heart failure was characterized by DNA fragmentation and by 3700 myocytes per million cells undergoing apoptotic cell death. This phenomenon was accompanied by 11,000 cells per million expressing Fas protein. Concurrently, 22 and 17 myocytes and interstitial cells per million showed nuclear mitotic division, whereas no changes in the relative proportions of mononucleated and multinucleated myocytes were detected. Moreover, PCNA-labeled myocytes accounted for 40,000 cells per million. CONCLUSIONS In conclusion, the induction of PCNA and Fas may be linked to the activation of myocyte proliferation and programmed cell death in the myocardium with rapid ventricular pacing, and these two cellular responses may play a key role in the development of the congestive dilated myopathy.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008938 Mitosis A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species. M Phase, Mitotic,Mitotic M Phase,M Phases, Mitotic,Mitoses,Mitotic M Phases,Phase, Mitotic M,Phases, Mitotic M
D009206 Myocardium The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow. Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D002304 Cardiac Pacing, Artificial Regulation of the rate of contraction of the heart muscles by an artificial pacemaker. Pacing, Cardiac, Artificial,Artificial Cardiac Pacing,Artificial Cardiac Pacings,Cardiac Pacings, Artificial,Pacing, Artificial Cardiac,Pacings, Artificial Cardiac
D002311 Cardiomyopathy, Dilated A form of CARDIAC MUSCLE disease that is characterized by ventricular dilation, VENTRICULAR DYSFUNCTION, and HEART FAILURE. Risk factors include SMOKING; ALCOHOL DRINKING; HYPERTENSION; INFECTION; PREGNANCY; and mutations in the LMNA gene encoding LAMIN TYPE A, a NUCLEAR LAMINA protein. Cardiomyopathy, Congestive,Congestive Cardiomyopathy,Dilated Cardiomyopathy,Cardiomyopathy, Dilated, 1a,Cardiomyopathy, Dilated, Autosomal Recessive,Cardiomyopathy, Dilated, CMD1A,Cardiomyopathy, Dilated, LMNA,Cardiomyopathy, Dilated, With Conduction Defect 1,Cardiomyopathy, Dilated, with Conduction Deffect1,Cardiomyopathy, Familial Idiopathic,Cardiomyopathy, Idiopathic Dilated,Cardiomyopathies, Congestive,Cardiomyopathies, Dilated,Cardiomyopathies, Familial Idiopathic,Cardiomyopathies, Idiopathic Dilated,Congestive Cardiomyopathies,Dilated Cardiomyopathies,Dilated Cardiomyopathies, Idiopathic,Dilated Cardiomyopathy, Idiopathic,Familial Idiopathic Cardiomyopathies,Familial Idiopathic Cardiomyopathy,Idiopathic Cardiomyopathies, Familial,Idiopathic Cardiomyopathy, Familial,Idiopathic Dilated Cardiomyopathies,Idiopathic Dilated Cardiomyopathy
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D004249 DNA Damage Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS. DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016276 Ventricular Function The hemodynamic and electrophysiological action of the HEART VENTRICLES. Function, Ventricular,Functions, Ventricular,Ventricular Functions

Related Publications

Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Stretch-induced programmed myocyte cell death.
November 1995, The Journal of clinical investigation,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Activation of cyclins and cyclin-dependent kinases, DNA synthesis, and myocyte mitotic division in pacing-induced heart failure in dogs.
December 1999, Laboratory investigation; a journal of technical methods and pathology,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Cell Death in the Cardiac Myocyte.
January 2015, Biological & pharmaceutical bulletin,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Effects of amiodarone on cardiac electrophysiology in right ventricular rapid pacing-induced heart failure dogs.
July 1998, Zhongguo yao li xue bao = Acta pharmacologica Sinica,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Developmental remodeling and shortening of the cardiac outflow tract involves myocyte programmed cell death.
October 1998, Development (Cambridge, England),
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Myocyte cell death and ventricular remodeling.
March 1997, Current opinion in nephrology and hypertension,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Efficacy of epicardial controlled-release lidocaine for ventricular tachycardia induced by rapid ventricular pacing in dogs.
November 1990, Journal of cardiovascular pharmacology,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Angiotensin II activates programmed myocyte cell death in vitro.
March 1997, Experimental cell research,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
End-stage cardiac failure in humans is coupled with the induction of proliferating cell nuclear antigen and nuclear mitotic division in ventricular myocytes.
December 1994, Circulation research,
Y Liu, and E Cigola, and W Cheng, and J Kajstura, and G Olivetti, and T H Hintze, and P Anversa
Myocyte mitotic division in the aging mammalian rat heart.
October 1991, Circulation research,
Copied contents to your clipboard!