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A nondefective recombinant human adenovirus 5 (Ad5) carrying the gene encoding for the porcine rotavirus outer capsid protein VP7 in the E3 region of the Ad5 genome has been obtained. mRNA for VP7 in recombinant Ad5KV14/VP7 infected cells was demonstrated by means of RT-PCR. Radioimmunoprecipitation of cell extracts infected with Ad5KV14/VP7 on early (12 h p.i.) and late (24 h p.i.) stages of adenovirus infection with rabbit polyclonal antiserum raised to purified rotavirus virions revealed a recombinant protein possessing the same electrophoretic mobility, 37 kDa, as the native rotavirus K VP7. One-dimensional peptide mapping also demonstrated the identity of the recombinant and native VP7.
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
November 2008,
Wei sheng wu xue bao = Acta microbiologica Sinica,
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
June 1994,
Journal of virology,
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
January 1988,
Nucleic acids research,
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
June 2016,
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases,
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
November 1992,
Virus genes,
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
June 2009,
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine],
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
January 2019,
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
April 2018,
Pathogens (Basel, Switzerland),
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
January 2012,
Iranian journal of public health,
K K Doronin, and
G K Iurov, and
N F Grinenko, and
A N Zakharchuk, and
T S Denisova, and
T M Baburina, and
V A Krugliak, and
B S Naroditskiĭ
January 1991,
The Journal of general virology,
