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Secreted pro-cathepsin B of HS-24 human lung-tumour cells, human alveolar macrophages and Wi-38 human lung-fibroblast cells was pre-purified by ion exchange chromatography and investigated by 2D gel electrophoresis. Four (Wi-38), six (HS-24) and ten (alveolar macrophages) polypeptides differing in charge, but with the same molecular mass of 45 kDa, were found. The isoelectrical points of these polypeptides ranged from 5.43 to 6.57. Deglycosylation reduced the mass (7 kDa) but did not change the charge pattern. This investigation established cell type-specific patterns of secreted pro-cathepsin B-forms, but only parts of these may be cell type-specific forms depending on differentiated expression of mRNA and post-translational modification.
B Werle, and
W Ebert, and
W Klein, and
E Spiess
September 1990,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
July 1997,
Clinical & experimental metastasis,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
January 1990,
Neoplasma,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
January 1989,
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
May 2010,
Journal of immunology (Baltimore, Md. : 1950),
B Werle, and
W Ebert, and
W Klein, and
E Spiess
January 2003,
Postepy higieny i medycyny doswiadczalnej,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
June 1990,
Nucleic acids research,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
March 1995,
International journal of cancer,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
July 1994,
Cancer,
B Werle, and
W Ebert, and
W Klein, and
E Spiess
September 1998,
Clinical chemistry and laboratory medicine,
