DNA flow cytometry in node-positive breast cancer. Prognostic value and correlation with morphologic and clinical factors. 1995

J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
Department of Obstetrics and Gynecology, Albert-Ludwigs-University, Freiburg, Germany.

OBJECTIVE To help clarify the possible usefulness of nuclear DNA content and S-phase fraction (SPF) as additional prognostic factors in node-positive breast cancer patients because there is increased interest in the development of new factors that might provide more detailed prognostic information. METHODS We performed a DNA and SPF analysis by flow cytometry using a multivariate statistical model on a group of 139 node-positive breast cancer patients with clearly defined inclusion and exclusion criteria. RESULTS The percentage of aneuploidy increased with the number of involved nodes. Aneuploid tumors were more often found among grade 3 and among receptor-negative tumors. Univariate analysis showed a strong effect on recurrence-free survival (RFS) for the number of involved nodes (P < .001) and for tumor size (P = .013). Grade 3 and receptor-negative tumors showed a nonsignificant trend toward increased risk. The relative risk of aneuploid tumors was 1.19 (95% confidence interval, 0.75-1.87). Multivariate analysis revealed only the number of involved nodes to be an independent prognostic factor (P = .002); ploidy showed no effect (P = .684). SPF did not show any significant effect on RFS, even in a univariate analysis. CONCLUSIONS These results suggest that nuclear DNA content and SPF correlate with morphologic factors. Their routine clinical use, however, in node-positive breast cancer patients receiving adjuvant therapy seems to have no clinical relevance and therefore can be omitted.

UI MeSH Term Description Entries
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D011379 Prognosis A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations. Prognostic Factor,Prognostic Factors,Factor, Prognostic,Factors, Prognostic,Prognoses
D001943 Breast Neoplasms Tumors or cancer of the human BREAST. Breast Cancer,Breast Tumors,Cancer of Breast,Breast Carcinoma,Cancer of the Breast,Human Mammary Carcinoma,Malignant Neoplasm of Breast,Malignant Tumor of Breast,Mammary Cancer,Mammary Carcinoma, Human,Mammary Neoplasm, Human,Mammary Neoplasms, Human,Neoplasms, Breast,Tumors, Breast,Breast Carcinomas,Breast Malignant Neoplasm,Breast Malignant Neoplasms,Breast Malignant Tumor,Breast Malignant Tumors,Breast Neoplasm,Breast Tumor,Cancer, Breast,Cancer, Mammary,Cancers, Mammary,Carcinoma, Breast,Carcinoma, Human Mammary,Carcinomas, Breast,Carcinomas, Human Mammary,Human Mammary Carcinomas,Human Mammary Neoplasm,Human Mammary Neoplasms,Mammary Cancers,Mammary Carcinomas, Human,Neoplasm, Breast,Neoplasm, Human Mammary,Neoplasms, Human Mammary,Tumor, Breast
D004171 Diploidy The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X. Diploid,Diploid Cell,Cell, Diploid,Cells, Diploid,Diploid Cells,Diploidies,Diploids
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000782 Aneuploidy The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1). Aneuploid,Aneuploid Cell,Aneuploid Cells,Aneuploidies,Aneuploids,Cell, Aneuploid,Cells, Aneuploid
D012189 Retrospective Studies Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons. Retrospective Study,Studies, Retrospective,Study, Retrospective

Related Publications

J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
January 1990, Cancer investigation,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
August 1992, The Indian journal of medical research,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
February 1988, Cancer,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
March 1992, The European journal of surgery = Acta chirurgica,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
January 1993, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
September 1993, Cancer,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
December 1992, Journal of clinical oncology : official journal of the American Society of Clinical Oncology,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
June 2006, Journal of clinical oncology : official journal of the American Society of Clinical Oncology,
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
July 1990, Archives of surgery (Chicago, Ill. : 1960),
J Pfisterer, and F Kommoss, and W Sauerbrei, and D Menzel, and M Kiechle, and E Giese, and M Hilgarth, and A Pfleiderer
August 1989, The New England journal of medicine,
Copied contents to your clipboard!