Biomaterial Database

Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D. 1995

T I Merkulova, and S G Abbasova, and A B Moshnikova

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia.

A panel of monoclonal antibodies (MAbs) directed against lipopolysaccharide (LPS) of Salmonella serogroups A, B, and D was generated. Nine most productive hybrid clones were selected from several fusions of mouse myeloma cells with splenocytes from BALB/c mice, immunized with the corresponding heat-killed bacteria. The MAbs were characterized by enzyme immunoassay, Western blot analysis, and dot-immunoblotting with LPS and whole bacteria of Salmonella serogroups A-E and some other representatives of the Enterobacteriaceae family. Seven MAbs were reactive with the sole Salmonella strain used as an immunogen; one MAb, SD:10D9H, reacted with the five major serogroups of Salmonella species (A, B, D, E1, and E2); and one MAb, SA:5D12A, reacted with Salmonella serogroups A-E and a rough strain of S. cholerae-suis. None of the MAbs reacted with LPS of E. coli 055:B5 or whole bacteria of E. coli K12, Klebsiella pneumoniae, or Proteus vulgaris. The typical ladder-like patterns of bands were observed after immunoblotting of MAbs against electrophoretically resolved LPS from Salmonella serogroups A-E, which thus confirmed their LPS-directed specificity. MAbs affinity constants were determined by noncompetitive enzyme immunoassay using serial dilutions of both LPS as antigen (coating the plate) and antibodies. On the base of the results obtained, the presumed epitopes for each of the MAbs were discussed. The usefulness of MAbs generated for diagnostic and protective purposes was declared.

UI	MeSH Term	Description	Entries
D008070	Lipopolysaccharides	Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)	Lipopolysaccharide,Lipoglycans
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000915	Antibody Affinity	A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.	Affinity, Antibody,Antibody Avidity,Avidity, Antibody,Affinities, Antibody,Antibody Affinities,Antibody Avidities,Avidities, Antibody
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012703	Serotyping	Process of determining and distinguishing species of bacteria or viruses based on antigens they share.	Serotypings