BIOMAT Database Logo BIOMAT Database Logo

Toxicity of sea nettle (Chrysaora quinquecirrha) fishing tentacle nematocyst venom in cultured rat hepatocytes. 1996

H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Department of Dermatology, University of Maryland Medical School, Baltimore, USA.

Sea nettle (Chrysaora quinquecirrha) venom (CQV) is known to be toxic to the cardiac, respiratory, renal and hepatic systems in animal models. However, the mechanism of toxicity of CQV on hepatocytes is unknown. We utilized isolated rat hepatocytes in culture and measured percentage total lactate dehydrogenase (LDH) release after direct exposure to CQV. Toxicity of CQV in this system produced a linear multiple-dose response curve as well as a linear single-dose kinetics curve. Neither extracellular calcium concentration nor intracellular calcium chelation had a statistically significant effect on the toxicity of CQV in our hepatocyte model. From these results it appears that CQV does not form large membrane channels similar to complement, nor does calcium appear to play a major role in the mechanism of toxicity in hepatocytes. The isolated rat hepatocyye culture and measure of LDH release provided a relatively simple and reproducible model for examining toxicity of CQV.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D007770 L-Lactate Dehydrogenase A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005397 Fish Venoms Venoms produced by FISHES, including SHARKS and sting rays, usually delivered by spines. They contain various substances, including very labile toxins that affect the HEART specifically and all MUSCLES generally. Fish Venom,Venom, Fish,Venoms, Fish
D000704 Analysis of Variance A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable. ANOVA,Analysis, Variance,Variance Analysis,Analyses, Variance,Variance Analyses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Sea nettle (Chrysaora quinquecirrha) nematocyst venom hemagglutinins.
January 1983, Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Sea nettle (Chrysaora quinquecirrha) nematocyst venom: mechanism of action on muscle.
January 1983, Toxicon : official journal of the International Society on Toxinology,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Characteristics of hyaluronidase and hemolytic activity in fishing tentacle nematocyst venom of Chrysaora quinquecirrha.
February 1994, Toxicon : official journal of the International Society on Toxinology,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Partial purification of sea nettle (Chrysaora quinquecirrha) nematocyst toxin.
March 1970, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Partial purification and characterization of the acid protease of sea nettle (Chrysaora quinquecirrha) nematocyst venom.
January 1982, Comparative biochemistry and physiology. B, Comparative biochemistry,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Effect of sea nettle (Chrysaora quinquecirrha) venom on isolated rat aorta.
January 1988, Toxicon : official journal of the International Society on Toxinology,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Human immunosuppression induced by sea nettle (Chrysaora quinquecirrha) venom.
January 1991, Toxicon : official journal of the International Society on Toxinology,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Immunosorbent chromatography of sea nettle (Chrysaora quinquecirrha) venom and characterization of toxins.
January 1983, Toxicon : official journal of the International Society on Toxinology,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
Antitumor and antioxidant role of Chrysaora quinquecirrha (sea nettle) nematocyst venom peptide against Ehrlich ascites carcinoma in Swiss Albino mice.
May 2010, Molecular and cellular biochemistry,
H E Houck, and M M Lipsky, and L Marzella, and J V Burnett
In vitro radical scavanging activities of Chrysaora quinquecirrha nematocyst venom.
April 2009, Drug discoveries & therapeutics,
Copied contents to your clipboard!