BIOMAT Database Logo BIOMAT Database Logo

Nuclear transfer in cattle using in vivo-derived vs. in vitro-produced donor embryos: effect of developmental stage. 1996

V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Bayerisches Forschungszentrum für Fortpflanzungsbiologie, Oberschleissheim, Munich, Germany.

To determine the best developmental stage of donor embryos for yielding the highest number of clones per embryo, we compared the efficiencies of nuclear transfer when using blastomeres from morulae or morulae at cavitation, or when using inner-cell-mass cells of blastocysts as nuclear donors. This comparison was done both on in vivo-derived and in vitro-produced donor embryos. In experiment 1, with in vivo-derived donor embryos, nuclei from morulae at cavitation supported the development of nuclear transfer embryos to the blastocyst stage (36%) at a rate similar to that of nuclei from morulae (27%), blastomeres from morulae at cavitation being superior (P < 0.05) to inner-cell-mass cells from blastocysts (21%). The number of blastocysts per donor embryo was significantly (P < 0.05) higher when using nuclei from morulae at cavitation (15.7 +/- 4.1) rather than nuclei from morulae (9.8 +/- 5.5) or blastocysts (6.3 +/- 3.3). With in vitro-produced donor embryos (experiment 2), nuclei from morulae yielded slightly more blastocysts (32%) than nuclei from morulae at cavitation (29%), both stages being superior to nuclei from blastocysts (15% development to the blastocyst stage). Morulae at cavitation yielded a higher number of cloned blastocysts per donor embryo (11.5 +/- 5.9) than did morulae (9.3 +/- 3.2) and blastocysts (3.3 +/- 1.4). Transfer of cloned embryos originating from in vivo-derived morulae, morulae at cavitation, and blastocysts resulted in four pregnancies (10%), three pregnancies (7%), and one (17%) pregnancy on day 45. The corresponding numbers of calves born were 3 (4%), 3 (7%), and 0, respectively. After transfer of blastocysts derived from in vitro nuclear donor morulae (n = 16) and morulae at cavitation (n = 7), two (20%) and two (50%) recipients, respectively, were pregnant on day 45. However, transfer of seven cloned embryos from in vitro donor blastocysts to three recipients did not result in a pregnancy. Using in vitro-produced donor embryos, calves were only obtained from morula-stage donors (13%). Our results indicate that the developmental stage of donor embryos affects the efficiency of nuclear transfer, with morulae at cavitation yielding a high number of cloned blastocysts.

UI MeSH Term Description Entries
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D001755 Blastocyst A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper. Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002999 Clone Cells A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed) Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D053652 Nuclear Transfer Techniques Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell. Nuclear Transplantation,Somatic Cell Nuclear Transfer Technique,Nuclear Transfer Technique,Transplantation, Nuclear

Related Publications

V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Improvement of the developmental ability of nuclear transfer embryos by using blastomeres from in vitro fertilized embryos selected according to the early developmental stage and cell division status as donor cells in cattle.
April 2011, The Journal of reproduction and development,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Developmental rate and ploidy of embryos produced by nuclear transfer with different activation treatments in cattle.
March 2006, Animal reproduction science,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Comparison of pregnancy rates with transfer of in vivo produced embryos derived using multiple ovulation and embryo transfer (MOET) with in vitro produced embryos by somatic cell nuclear transfer (SCNT) in the dromedary camel (Camelus dromedaries).
October 2019, Animal reproduction science,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Developmental competence and gene expression in preimplantation bovine embryos derived from somatic cell nuclear transfer using different donor cells.
August 2005, Zygote (Cambridge, England),
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Developmental competence of 8?16-cell stage bison embryos produced by interspecies somatic cell nuclear transfer.
March 2015, Reproduction, fertility, and development,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Effect of synchronization of donor cells in early G1-phase using shake-off method on developmental potential of somatic cell nuclear transfer embryos in cattle.
August 2013, Animal science journal = Nihon chikusan Gakkaiho,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Transfer of inner cell mass cells derived from bovine nuclear transfer embryos into the trophoblast of bovine in vitro-produced embryos.
January 2006, Cloning and stem cells,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Effect of the nuclear-donor cell lineage, type, and cell donor on development of somatic cell nuclear transfer embryos in cattle.
January 2005, Cloning and stem cells,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
In vitro developmental potential of bovine nuclear transfer embryos derived from primary cultured cumulus cells.
March 2000, The Journal of veterinary medical science,
V Zakhartchenko, and H D Reichenbach, and J Riedl, and G A Palma, and E Wolf, and G Brem
Effect of donor cell age on development of ovine nuclear transfer embryos in vitro.
November 2010, Zygote (Cambridge, England),
Copied contents to your clipboard!