Immunosuppressive activity of 15-deoxyspergualin on normal and autoimmune peripheral blood mononuclear cells. 1996

F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
Institute of Internal Medicine, Infectious Diseases and Immunopathology, University of Milan, Italy.

Several experimental conditions were used in this study to evaluate the in vitro effects of 15-deoxyspergualin on the function of T lymphocytes, B lymphocytes and monocytes from healthy subjects and patients suffering from systemic lupus erythematosus. Whilst the secretion of polyclonal immunoglobulin (Ig) M and IgG from the B lymphocytes of the healthy subjects was diminished by 15-deoxyspergualin, neither the proliferative response of normal T and B cells to mitogenic stimulation nor the cytokine secretory capacity of these cells (e.g. interleukin-2, -4, -6 and gamma-interferon) and monocytes (e.g. interleukin-1 beta and -6) were affected by the drug. In contrast, on the mononuclear cells obtained from the lupus patients not only did 15-deoxyspergualin inhibit the spontaneous production of polyclonal and anti-DNA IgG antibodies but also suppressed interleukin-1 beta secretion from the monocytes. Other functional responses of T and B cells and monocytes from lupus patients, including mitogenic activation and cytokine secretion, were not altered by the drug. These data suggest that 15-deoxyspergualin possesses a novel mechanism of pharmacological immunosuppression apparently different from that of other immunosuppressants, such as cyclosporin A, FK506 and corticosteroids, that seems to be primarily displayed at the level of autoreactive B cells and monocytes.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007075 Immunoglobulin M A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin. Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007166 Immunosuppressive Agents Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging. Immunosuppressant,Immunosuppressive Agent,Immunosuppressants,Agent, Immunosuppressive,Agents, Immunosuppressive
D008180 Lupus Erythematosus, Systemic A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. Libman-Sacks Disease,Lupus Erythematosus Disseminatus,Systemic Lupus Erythematosus,Disease, Libman-Sacks,Libman Sacks Disease
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D009000 Monocytes Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles. Monocyte
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D006146 Guanidines A family of iminourea derivatives. The parent compound has been isolated from mushrooms, corn germ, rice hulls, mussels, earthworms, and turnip juice. Derivatives may have antiviral and antifungal properties.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
May 1986, The Journal of experimental medicine,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
January 1991, Drugs under experimental and clinical research,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
January 1993, Agents and actions,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
February 1992, Transplantation proceedings,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
February 1989, Transplantation proceedings,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
November 1987, The Journal of antibiotics,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
December 1999, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
September 1992, Transplant international : official journal of the European Society for Organ Transplantation,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
April 1987, The Journal of antibiotics,
F Nicoletti, and M O Borghi, and W Barcellini, and C Fain, and B Beltrami, and N Del Papa, and H U Schorlemmer, and L Mottola, and P L Meroni
January 1992, Veterinary immunology and immunopathology,
Copied contents to your clipboard!