The effect of removal of the pituitary on mesenteric arterial function was examined in adult male rats that had undergone hypophysectomy 7 days earlier. Sham-operated rats and weight-matched rats served as controls. The body weight of hypophysectomized rats decreased from 220.75 +/- 0.48 g to 188.03 +/- 2.53 g (n = 7). Sham-operated controls gained weight, from 223 +/- 1.47 to 275.85 +/- 3.45 g (n = 8). Frequency-dependent vasoconstriction to electrical field stimulation (2-32 Hz, 90 V, 1 ms, 30 s) was significantly augmented after hypophysectomy. The maximal constrictor response of hypophysectomized preparations, 215.5 +/- 14.9 mm Hg (n = 6), was approximately twice that of the sham-operated controls, 100 +/- 6.1 mm Hg (n = 7) and weight-matched controls 109.8 +/- 5.8 mm Hg (n = 8). Norepinephrine (NE) (0.05-1,500 nmol) elicited dose-dependent vasoconstriction; the maximal response was significantly augmented after hypophysectomy, 221.71 +/- 15.9 (n = 7) as compared with 148.0 +/- 16.0 mm Hg (n = 8) in sham-operated controls and 146.3 +/- 8.7 mm Hg (n = 7) in weight-matched controls. Dose-dependent vasoconstrictor responses to ATP, 5-hydroxytryptamine, vasopressin, and endothelin were similar between the groups. High-performance liquid chromatography analysis showed no difference in NE content of the superior mesenteric artery from hypophysectomized and sham-operated controls. Hypophysectomy of rats caused an increase in sympathetic constrictor function of the mesenteric arterial vasculature that appeared to involve postjunctional adrenoceptors rather than prejunctional mechanisms and was not due to arrested growth or the smaller size of the mesenteric preparations.