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Platelet membrane glycoproteins play a central role in platelet aggregation and thus in primary hemostasis. To investigate mechanisms of platelet-platelet interaction in the absence of cellular activation events, we studied immunological and functional aspects of isolated platelet membranes. Platelet membranes contained significant amounts of the inducible fibrinogen receptor, glycoprotein (GP) IIb-IIIa, which exposes conformation-dependent LIBS1 and PMI-1 epitopes in response to fibrinogen-mimetic peptides GRGDSP and HHLG-GAKQAGDV. In the presence of soluble fibrinogen, membrane-coated latex beads showed Ca(2+)-dependent agglutination that could be partially inhibited by GRGDSP but not by the biologically inactive peptide GRGESP. Thrombospondin enhanced agglutination of membrane-coated beads, which could be inhibited by polyvalent anti-thrombospondin Fab fragments and anti-thrombospondin monoclonal antibody MA-II. Mg2+ inhibited both GPIIb-IIIa- and thrombospondin-mediated agglutination of membranes in a dose-dependent manner. The results of the present study indicate that isolated platelet membranes are a useful tool to study regulation of GPIIb-IIIa- and thrombospondin-mediated platelet-platelet interaction.
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
December 1980,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
January 1967,
Thrombosis et diathesis haemorrhagica. Supplementum,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
June 1978,
FEBS letters,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
December 1974,
Thrombosis et diathesis haemorrhagica,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
January 1967,
Thrombosis et diathesis haemorrhagica. Supplementum,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
January 1967,
Bibliotheca anatomica,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
June 1955,
Archives internationales de physiologie et de biochimie,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
January 1983,
Haemostasis,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
November 1975,
Thrombosis et diathesis haemorrhagica,
M Gawaz, and
I Ott, and
A J Reininger, and
U Heinzmann, and
F J Neumann
December 1966,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
