Biomaterial Database

Boldine prevents human liver microsomal lipid peroxidation and inactivation of cytochrome P4502E1. 1995

P Kringstein, and A I Cederbaum

Department of Biochemistry, Mount Sinai School of Medicine, New York, NY 10029, USA.

Boldine, an alkaloid found in the leaves and bark of boldo, prevented the ferric-ATP catalyzed peroxidation of human liver microsomes. Lipid peroxidation, dependent upon electron transfer from NADPH or NADH, was comparably inhibited by boldine, with a K(I) value of about 5 microM. Inactivation and decreased content of human cytochrome P4502E1 as a consequence of incubating microsomes with ferric-ATP and reductant was completely prevented by boldine. However, inactivation of cytochrome P4502E1 by CCl4 was not prevented by boldine, although the alkaloid prevented CCl4-catalyzed lipid peroxidation. This suggests that the CCl4 inactivation of P4502E1 may be independent of CCl4-mediated lipid peroxidation. In view of its low toxicity, lack of effect on P450 activity, and strong inhibition of peroxidation of human liver microsomes, boldine may be valuable as an antioxidant and hepatoprotective agent.

UI	MeSH Term	Description	Entries
D007501	Iron	A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.	Iron-56,Iron 56
D008862	Microsomes, Liver	Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.	Liver Microsomes,Liver Microsome,Microsome, Liver
D002251	Carbon Tetrachloride	A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed)	Tetrachloromethane,Tetrachloride, Carbon
D004791	Enzyme Inhibitors	Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.	Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000975	Antioxidants	Naturally occurring or synthetic substances that inhibit or retard oxidation reactions. They counteract the damaging effects of oxidation in animal tissues.	Anti-Oxidant,Antioxidant,Antioxidant Activity,Endogenous Antioxidant,Endogenous Antioxidants,Anti-Oxidant Effect,Anti-Oxidant Effects,Anti-Oxidants,Antioxidant Effect,Antioxidant Effects,Activity, Antioxidant,Anti Oxidant,Anti Oxidant Effect,Anti Oxidant Effects,Anti Oxidants,Antioxidant, Endogenous,Antioxidants, Endogenous
D001060	Aporphines	Dibenzoquinolines derived in plants from (S)-reticuline (BENZYLISOQUINOLINES).
D015227	Lipid Peroxidation	Peroxidase catalyzed oxidation of lipids using hydrogen peroxide as an electron acceptor.	Lipid Peroxidations,Peroxidation, Lipid,Peroxidations, Lipid
D065691	Cytochrome P-450 CYP2E1 Inhibitors	Drugs and compounds which inhibit or antagonize the biosynthesis or actions of CYTOCHROME P-450 CYP2E1.	CYP2E1 Inhibitors,P450 CYP2E1 Inhibitors,CYP2E1 Inhibitors, P450,Cytochrome P 450 CYP2E1 Inhibitors,Inhibitors, CYP2E1,Inhibitors, P450 CYP2E1
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In