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A rapid method is described for the determination of some steroidal hormones in adrenal tissue. The following steroids were measured: pregnenolone, progesterone, deoxycorticosterone, corticosterone, aldosterone, 17-alpha-OH-pregnenolone, deoxycortisol, cortisol, cortisone, 17-alpha-OH-progesterone, dehydroepiandrosterone, androstendione, and testosterone. After extraction of the steroids the purification steps were performed by thin layer chromatography. Gas chromatography was used for further separation and quantitative analysis of underivatized steroids. The GC-analysis of steroids without any derivatisation makes this procedure comparatively simple and exact. Recovery of the steroid content of the tissue ranged from 30% to 70%. This method described herewith has several advantages, and allows the analysis of two tissues at the same time for a large number of adrenal steroids within two weeks.
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1976,
Arzneimittel-Forschung,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1981,
Arzneimittel-Forschung,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1952,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
August 1973,
Archiv fur Toxikologie,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1980,
Journal de pharmacie de Belgique,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1977,
Arzneimittel-Forschung,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
October 1976,
Archives of toxicology,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1963,
Voprosy meditsinskoi khimii,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
October 1981,
Casopis lekaru ceskych,
K Herkner, and
J Jörg, and
W Waldhäusl, and
H Haschek
January 1981,
Journal de pharmacie de Belgique,
