-transparent.png){kind=logo}
PMEA [9-(2-phosphonylmethoxyethyl)adenine] inhibited both HSV-1 and HIV-1 replication in MT-2 and HeLa-CD4 cells. (S)-1-[3-hydroxy-2-(phosphonylmethoxy)propyl]cytosine (HPMPC) inhibited both these viruses in the epithelioid HeLa-CD4 cells, but did not inhibit either virus in the T-lymphocytic MT-2 cells. PMEA and HPMPC are metabolized to their diphosphorylated forms within cells, which then inhibit viral polymerases. We therefore compared the metabolism of PMEA and HPMPC in MT-2 and HeLa CD4 cells. PMEApp formation was efficient in both the cell types, whereas HPMPCpp levels were approximately 3-10 fold lower in MT-2 cells, compared to HeLa-CD4 cells. These results indicate that HPMPC can inhibt HIV replication in the appropriate cell types, and show that differences in their metabolism cannot account entirely for the lack of antiviral efficacy of HPMPC in MT-2 cells.
R V Srinivas, and
M Connely, and
A Fridland
September 1993,
Biochemical pharmacology,
R V Srinivas, and
M Connely, and
A Fridland
July 1991,
Antiviral research,
R V Srinivas, and
M Connely, and
A Fridland
May 1990,
Antiviral research,
R V Srinivas, and
M Connely, and
A Fridland
May 1992,
Acta virologica,
R V Srinivas, and
M Connely, and
A Fridland
December 1988,
Antimicrobial agents and chemotherapy,
R V Srinivas, and
M Connely, and
A Fridland
February 2000,
Nippon Ganka Gakkai zasshi,
R V Srinivas, and
M Connely, and
A Fridland
October 1991,
Acta crystallographica. Section C, Crystal structure communications,
R V Srinivas, and
M Connely, and
A Fridland
November 1990,
Virology,
R V Srinivas, and
M Connely, and
A Fridland
January 1990,
Advances in experimental medicine and biology,
R V Srinivas, and
M Connely, and
A Fridland
November 1992,
Cornea,
