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Crystallization and preliminary X-ray analysis of a truncated family A alkaline endoglucanase isolated from Bacillus sp. KSM-635. 1997
T Shirai, and
T Yamane, and
T Hidaka, and
K Kuyama, and
A Suzuki, and
T Ashida, and
K Ozaki, and
S Ito
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University.
The catalytic domain of an alkaline endo-1,4-beta-glucanase (family A) isolated from Bacillus sp. KSM-635 (Mr = 40.2 kDa) was crystallized using the hanging drop vapor diffusion method. Two different crystal forms were obtained. Form 2 crystals (trigonal space group R3 with cell dimensions of a = b = 111.9 and c = 207.1 angstroms in a hexagonal lattice) were found to be more stable than form 1 ones upon X-ray irradiation. A full data set for form 2 crystals has been collected up to 3.3 angstroms resolution.
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