Biomaterial Database

Studies on nucleotide diphosphate diacylglycerol specificity of acidic phospholipid biosynthesis in rat liver subcellular fractions. 1976

B J Poorthuis, and K Y Hostetler

(1)Cytidine diphosphate diacylglycerol, uridine diphosphate diacylglycerol, adenosine diphosphate diacylglycerol and guanosine diphosphate diacylglycerol were synthesized chemically and their purity assessed. The acticity of these compounds in acidic phospholipid synthesis was examined in rat liver mitochondria and microsomes. (2) Phosphatidylglycerol synthesis in rat liver mitochondria exhibited considerable activity with CDP diacylglycerol (v 7.0 nmol mg-1 h-1). UDPdiacylglycerol (v 5.4) and ADP diacylglyc erol (v 4.2). GDP diacylglycerol activity was detectable but very low. (3) Diphophatidylglycerol formation in mitochondria and phosphatidylinositolsythesis in microsomes exhibited considerable more specificity for CDP diacylglycerol. However, at high concentrations, measurable diphosphaticylglycerol and phophatidylinositol synthesis was observed with the other liponucleotides. (4) Although considerable phosphatidylglycerol formation was observed with UDPdiacaylglycerol and ADP diacylglycerol, it is unlikelky that these compounds are of physiologic importance, at least in rat liver, since CTP:phophatidic acid cytidyltransferase in microsomes and mitochondria was shown to be specific for cytidine triphosphate. The lack of specificity of phosphatiidylglycerol synthesis for CDP diacylglycerol is currently unexplained but may be of some importance in other tissues or in other organisms.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008862	Microsomes, Liver	Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.	Liver Microsomes,Liver Microsome,Microsome, Liver
D008930	Mitochondria, Liver	Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)	Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D009702	Nucleoside Diphosphate Sugars		Diphosphate Sugars, Nucleoside,Sugars, Nucleoside Diphosphate
D010712	Phosphatidic Acids	Fatty acid derivatives of glycerophosphates. They are composed of glycerol bound in ester linkage with 1 mole of phosphoric acid at the terminal 3-hydroxyl group and with 2 moles of fatty acids at the other two hydroxyl groups.	Ammonium Phosphatidate,Diacylglycerophosphates,Phosphatidic Acid,Acid, Phosphatidic,Acids, Phosphatidic,Phosphatidate, Ammonium
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D003567	Cytidine Diphosphate Diglycerides	The ester of diacylglycerol with the terminal phosphate of cytidine diphosphate. It serves as an intermediate in the biosynthesis of phosphatidylethanolamine and phosphatidylserine in bacteria.	CDP Diacylglycerols,CDP Diglycerides,Cytidine Diphosphodiacylglycerols,CDP-Diacylglycerol,CDP Diacylglycerol,Diacylglycerols, CDP,Diglycerides, CDP,Diglycerides, Cytidine Diphosphate,Diphosphate Diglycerides, Cytidine,Diphosphodiacylglycerols, Cytidine
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013347	Subcellular Fractions	Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)	Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction