Biomaterial Database

In vitro incorporation of isomeric cis-octadecenoic acids by rat liver mitochondria. 1976

D Sgoutas, and R Jones, and P Befanis

The metabolic fate of positional isomers of cis-octadecenoic acids was compared to that of oleic acid, elaidic acid and stearic acid in rat liver mitochondria. The positional isomers as well as elaidic acid and stearic acid were labelled with 3H and they were incubated in pairs with [1-14C]oleic acid. 3H/14C ratios were determined for the administered mixtures and for the isolated lipid classes. The results suggested that all isomers were readily incorporated into the membraneous structure of mitochondria. Those with the double bond in the middle of the acyl chain resembled oleic acid and they were preferentially incorporated in cholesterol esters, triacylglycerols and in the 2-position of triacylglycerols and of phosphatidylcholines. Those with the double bond away from the middle of the chain were metabolically distinct from oleic acid and behaved like trans fatty acids. They were rapidly taken up by mitochondria. They were preferentially incorporated in phospholipids and they occupied the 1-position in phosphatidylcholines and the 1- and 3-positions in triacylglycerols.

UI	MeSH Term	Description	Entries
D007536	Isomerism	The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)	Isomerisms
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008930	Mitochondria, Liver	Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)	Liver Mitochondria,Liver Mitochondrion,Mitochondrion, Liver
D010713	Phosphatidylcholines	Derivatives of PHOSPHATIDIC ACIDS in which the phosphoric acid is bound in ester linkage to a CHOLINE moiety.	Choline Phosphoglycerides,Choline Glycerophospholipids,Phosphatidyl Choline,Phosphatidyl Cholines,Phosphatidylcholine,Choline, Phosphatidyl,Cholines, Phosphatidyl,Glycerophospholipids, Choline,Phosphoglycerides, Choline
D005231	Fatty Acids, Unsaturated	FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.	Fatty Acids, Polyunsaturated,Polyunsaturated Fatty Acid,Unsaturated Fatty Acid,Polyunsaturated Fatty Acids,Acid, Polyunsaturated Fatty,Acid, Unsaturated Fatty,Acids, Polyunsaturated Fatty,Acids, Unsaturated Fatty,Fatty Acid, Polyunsaturated,Fatty Acid, Unsaturated,Unsaturated Fatty Acids
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014280	Triglycerides	An ester formed from GLYCEROL and three fatty acid groups.	Triacylglycerol,Triacylglycerols,Triglyceride
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus