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We have previously demonstrated that hydrogen peroxide (H2O2) treatment of bovine tracheal myocytes increases the activity of extracellular signal-regulated kinases (ERK), serine/threonine kinases of the mitogen-activated protein (MAP) kinase superfamily thought to play a key role in the transduction of mitogenic signals to the cell nucleus. Moreover, H2O2-induced ERK activation was partially reduced by pretreatment with phorbol 12,13-dibutyrate, which depletes protein kinase C (PKC). In this study, we further examined the signaling intermediates responsible for ERK activation by H2O2 in airway smooth muscle, focusing on MAP kinase/ERK kinase (MEK), a dual-function kinase which is required and sufficient for ERK activation in bovine tracheal myocytes; Raf-1, a serine/threonine kinase known to activate MEK; and PKC. Pretreatment of cells with inhibitors of MEK (PD98059), Raf-1 (forskolin), and PKC (chelerythrine) each reduced H2O2-induced ERK activity. In addition, H2O2 treatment significantly increased both MEK1 and Raf-1 activity. No activation of MEK2 was detected. Together these data suggest that H2O2 may stimulate ERK via successive activation of PKC, Raf-1, and MEK1.
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
August 2001,
Proceedings of the National Academy of Sciences of the United States of America,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
October 1993,
The Journal of experimental medicine,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
March 2005,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
February 2010,
Clinical and experimental pharmacology & physiology,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
January 2001,
Inflammation research : official journal of the European Histamine Research Society ... [et al.],
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
November 2009,
Molecular pharmacology,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
October 2003,
Experimental cell research,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
August 2013,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
August 2000,
The Journal of biological chemistry,
M K Abe, and
S Kartha, and
A Y Karpova, and
J Li, and
P T Liu, and
W L Kuo, and
M B Hershenson
June 2007,
Molecular pharmacology,
