Biomaterial Database

Ribosomal RNA synthesis in the mycelium of Phycomyces blakesleeanus. High molecular weight RNA. 1976

J A Pearson, and K Thomas

Total RNA was extracted from 24 h old mycelia of Phycomyces blakesleeanus which had been grown in liquid culture. Heavy molecular weight RNA was fractionated on 2.6% polyacrylamide gels. 1. The main cytoplasmic ribosomal RNA fractions had molecular weights of 1.34 and 0.72-16(6). 2. [3H]Uridine pulse labelling of cultures revealed an initial ribosomal RNA precursor of molecular weight 2.5-10(6). 3. An intermediate precursor for the 1.34 - 10(6) dalton peak was found, having a molecular weight of 1.6-10(6). 4. Pulse-chase experiments, using [3H]methionine, showed the flow of synthesis as being (see article). 5. Mature 0.72-10(6) dalton RNA appeared prior to the 1.34-10(6) dalton RNA but no immediate precursor for it was detected.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D010800	Phycomyces	A genus of zygomycetous fungi in the family Mucoraceae, order MUCORALES, forming mycelia having a metallic sheen. It has been used for research on phototropism.	Phycomyce
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005658	Fungi	A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.	Fungi, Filamentous,Molds,Filamentous Fungi,Filamentous Fungus,Fungus,Fungus, Filamentous,Mold
D012335	RNA, Ribosomal	The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)	Ribosomal RNA,15S RNA,RNA, 15S
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription