Biomaterial Database

Sequence studies on post-ecdysial cuticular proteins from pupae of the yellow mealworm, Tenebrio molitor. 1998

D Baernholdt, and S O Anderson

August Krogh Institute, Copenhagen University, Denmark.

Proteins were extracted from the cuticle mid-instar pupae of Tenebrio and purified by column chromatography. The protein pattern obtained by two-dimensional gel-electrophoresis was different from that obtained from pharate pupal cuticle, indicating that Tenebrio during the post-ecdysial pupal deposits cuticular proteins different from those deposited during the preecdysial period. The complete amino acid sequence was determined for four of the urea-extractable proteins from Tenebrio midinstar pupal cuticle. They range from 5.8 to 16.7 kDa in molecular weights and from 5.2 to 7.9 in isoelectric points. Little similarity was observed between the sequenced post-and pre-ecdysial cuticular proteins from Tenebrio pupae. Only one of the sequenced post-ecdysial proteins contains the Ala-Ala-Pro-Ala/Val motif common in proteins from Tenebrio larval/pupal pharate cuticle and from locust pharate cuticle. None of the post-ecdysial proteins contains the conserved hydrophilic sequence regions described for Tenebrio pharate cuticular proteins.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011679	Pupa	An inactive stage between the larval and adult stages in the life cycle of INSECTA.	Chrysalis,Pupae
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013712	Tenebrio	A genus of beetles which infests grain products. Its larva is called mealworm.	Mealworm,Mealworms,Tenebrios
D017421	Sequence Analysis	A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.	Sequence Determination,Analysis, Sequence,Determination, Sequence,Determinations, Sequence,Sequence Determinations,Analyses, Sequence,Sequence Analyses
D018978	Molting	Periodic casting off FEATHERS; HAIR; or cuticle. Molting is a process of sloughing or desquamation, especially the shedding of an outer covering and the development of a new one. This phenomenon permits growth in ARTHROPODS, skin renewal in AMPHIBIANS and REPTILES, and the shedding of winter coats in BIRDS and MAMMALS.	Ecdysis,Moulting,Ecdyses,Moltings,Moultings
D019476	Insect Proteins	Proteins found in any species of insect.	Insect Protein,Protein, Insect,Proteins, Insect