-transparent.png){kind=logo}
Mannan-binding protein (MBP) is a calcium-dependent mammalian serum lectin important in first-line host defense. MBP belongs to the collectin family, which is characterized by an NH2-terminal cysteine-rich domain, a collagen-like domain, a neck domain, and a carbohydrate recognition domain (CRD). We have expressed a recombinant human MBP, consisting of the short collagen region (two repeats of Gly-Xaa-Yaa amino acid sequences), the neck domain, and the CRD, in Escherichia coli. The truncated MBP was capable of forming trimers by association of the neck domain and could bind sugar with a specificity similar to that of the native form. Results of hemagglutination inhibition (HI) assay of influenza A virus showed that the truncated MBP inhibited hemagglutination less strongly, although the native MBP induced the HI phenomenon. These results suggest that an oligomeric structure is an advantage for MBP to have full biological activity against influenza A virus.
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
October 2006,
Protein expression and purification,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
January 2022,
Journal of advanced pharmaceutical technology & research,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
July 2005,
Biotechnology letters,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
January 2004,
Glycoconjugate journal,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
January 2003,
Anticancer research,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
March 1991,
Journal of biochemistry,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
October 1987,
The Journal of biological chemistry,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
December 1992,
Biochemistry,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
April 2005,
Biochimica et biophysica acta,
S Eda, and
Y Suzuki, and
T Kawai, and
K Ohtani, and
T Kase, and
T Sakamoto, and
N Wakamiya
February 1993,
The Journal of biological chemistry,
