-transparent.png){kind=logo}
We have probed the surface accessibility of residues alpha187 to alpha199 of the Torpedo acetylcholine receptor with monoclonal antibody 383C, which binds uniquely to these residues. However, 383C binds to only one of the two alpha subunits in the membrane-bound receptor, neither of the two subunits in carbamylcholine-desensitized receptor, and to both alpha subunits in Triton X-100 solubilized receptor. The kinetics of association and dissoci-ation of 383C with the peptide alpha(183-199) compared to those with the membrane-bound receptor suggest that all but a single hydrogen bond of affinity derives from contacts between this peptide and the monoclonal antibody paratope. Inhibition of 383C binding by alpha-bungarotoxin selectively directed to the alpha subunit correlated with the high-affinity d-tubocurarine binding site, along with a lack of inhibition by alpha-bungarotoxin directed to the alpha subunit correlated with the low-affinity d-tubocurarine binding site, suggests that the 383C epitope on the membrane-bound receptor resides on the alpha subunit associated with the high-affinity d-tubocurarine binding site. The results presented here suggest a structural basis for the differences between the two receptor acetylcholine binding sites.
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
October 1985,
The Biochemical journal,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
April 1983,
Nature,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
August 1987,
Proceedings of the National Academy of Sciences of the United States of America,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
May 1982,
Biochemical and biophysical research communications,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
May 1984,
Proceedings of the National Academy of Sciences of the United States of America,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
May 1982,
FEBS letters,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
June 1983,
The Journal of biological chemistry,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
January 1979,
Biochemistry,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
October 1980,
Proceedings of the National Academy of Sciences of the United States of America,
R H Fairclough, and
G M Twaddle, and
E Gudipati, and
M Y Lin, and
D P Richman
March 1986,
Science (New York, N.Y.),
