Biomaterial Database

Dynamics and ordering in mixed model membranes of dimyristoylphosphatidylcholine and dimyristoylphosphatidylserine: a 250-GHz electron spin resonance study using cholestane. 1998

J P Barnes, and J H Freed

Baker Laboratory of Chemistry, Cornell University, Ithaca, New York 14853-1301, USA.

We report here on a 250-GHz electron spin resonance (ESR) study of macroscopically aligned model membranes composed of mixtures of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylserine (DMPS), utilizing the nixtroxide-labeled cholesterol analog cholestane (CSL). Two clearly resolved spectral components, distinct in both their ordering and dynamics, are resolved. The major component in membranes composed mostly of DMPC shows typical characteristics, with the long axis of CSL parallel to the bilayer normal with slow (10(6) </= R </= 10(7) s-1) rotational diffusion rates, as expected for cholesterol. The second component grows in as the mole fraction of DMPS increases. A detailed analysis shows that CSL senses a local, strongly biaxial environment. Our results imply that the inefficient packing between cholesterol and DMPS occurs probably because of the strong interactions between the PS headgroups, which provide the local biaxiality. Such a packing of the headgroups has been predicted by molecular dynamics simulations but had not been observed experimentally. The analysis of these spectral components was greatly aided by the excellent orientational resolution provided by the 250-GHz spectra. This enabled the key qualitative features of this interpretation to be "read" off the spectra before the detailed analysis.

UI	MeSH Term	Description	Entries
D008051	Lipid Bilayers	Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.	Bilayers, Lipid,Bilayer, Lipid,Lipid Bilayer
D008968	Molecular Conformation	The characteristic three-dimensional shape of a molecule.	Molecular Configuration,3D Molecular Structure,Configuration, Molecular,Molecular Structure, Three Dimensional,Three Dimensional Molecular Structure,3D Molecular Structures,Configurations, Molecular,Conformation, Molecular,Conformations, Molecular,Molecular Configurations,Molecular Conformations,Molecular Structure, 3D,Molecular Structures, 3D,Structure, 3D Molecular,Structures, 3D Molecular
D011336	Probability	The study of chance processes or the relative frequency characterizing a chance process.	Probabilities
D002776	Cholestanes	Derivatives of the saturated steroid cholestane with methyl groups at C-18 and C-19 and an iso-octyl side chain at C-17.
D004058	Diffusion	The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.	Diffusions
D004134	Dimyristoylphosphatidylcholine	A synthetic phospholipid used in liposomes and lipid bilayers for the study of biological membranes.	Dimyristoyllecithin,1,2-Dimyristoyl-glycero-3-phosphorylcholine,1,2-Ditetradecanoyl-glycero-3-phosphocholine,1,2-Ditetradecyl-glycero-3-phosphocholine,DMCP,DMPC,1,2 Dimyristoyl glycero 3 phosphorylcholine,1,2 Ditetradecanoyl glycero 3 phosphocholine,1,2 Ditetradecyl glycero 3 phosphocholine
D004578	Electron Spin Resonance Spectroscopy	A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.	ENDOR,Electron Nuclear Double Resonance,Electron Paramagnetic Resonance,Paramagnetic Resonance,Electron Spin Resonance,Paramagnetic Resonance, Electron,Resonance, Electron Paramagnetic,Resonance, Electron Spin,Resonance, Paramagnetic
D013113	Spin Labels	Molecules which contain an atom or a group of atoms exhibiting an unpaired electron spin that can be detected by electron spin resonance spectroscopy and can be bonded to another molecule. (McGraw-Hill Dictionary of Chemical and Technical Terms, 4th ed)	Spin Label,Label, Spin,Labels, Spin
D014461	Ultracentrifugation	Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D014494	Unithiol	A chelating agent used as an antidote to heavy metal poisoning.	2,3-Dimercapto-1-propanesulfonic Acid,2,3-Dimercaptopropane 1-Sodium Sulfonate,2,3-Dimercaptopropane-1-sulfonate Sodium,2,3-Dimercaptopropanesulfonic Acid,DMPS,DMPS-Heyl,Dimaval,Mercuval,Sodium 2,3-Dimercaptopropane Sulfonate,Sodium 2,3-Dithiopropanesulfate,Unitiol,1-Sodium Sulfonate, 2,3-Dimercaptopropane,2,3 Dimercapto 1 propanesulfonic Acid,2,3 Dimercaptopropane 1 Sodium Sulfonate,2,3 Dimercaptopropane 1 sulfonate Sodium,2,3 Dimercaptopropanesulfonic Acid,2,3-Dimercaptopropane Sulfonate, Sodium,2,3-Dithiopropanesulfate, Sodium,DMPS Heyl