Biomaterial Database

Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus. 1998

M Moreno, and J J Bernal, and I Jiménez, and E Rodríguez-Cerezo

Centro Nacional de Biotecnología (CSIC), Campus de Cantoblanco, Madrid, Spain.

Tobacco plants were transformed with genes encoding the tobacco vein mottling potyvirus (TVMV) P1 or P3 protein. When compared with vector-transformed or P1 transgenic lines, seedlings of P3 transgenic lines (except a low expressor line) developed poorly, suggesting a detrimental effect of P3 on the plant. All P1 and P3 transgenic lines were protected against the homologous TVMV strain and showed variable proportions of two resistance phenotypes: asymptomatic plants or symptomatic plants that recovered from infection. The resistance was effective against a high inoculum dose but had a narrow spectrum. The heterologous strain TVMV-S was able to overcome resistance in most P1 lines but did not break the resistance of most P3 lines. No line showed resistance to another potyvirus (potato virus Y) or to potato virus X. These features and the low levels of transgene expression in resistant plants suggest that protection in P1 and P3 lines is RNA-mediated. In contrast with most reports on virus-activated gene silencing, some P3 lines with the predominant 'recovery' phenotype showed silencing of the transgene that was activated at a certain developmental stage of the plant independently of virus infection.

UI	MeSH Term	Description	Entries
D010947	Plants, Toxic	Plants or plant parts which are harmful to man or other animals.	Plants, Poisonous,Plant, Poisonous,Plant, Toxic,Poisonous Plant,Poisonous Plants,Toxic Plant,Toxic Plants
D005814	Genes, Viral	The functional hereditary units of VIRUSES.	Viral Genes,Gene, Viral,Viral Gene
D014026	Nicotiana	A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; the dried leaves of Nicotiana tabacum are used for SMOKING.	Tobacco Plant,Nicotiana tabacum,Plant, Tobacco,Plants, Tobacco,Tobacco Plants
D014764	Viral Proteins	Proteins found in any species of virus.	Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D017800	Potyvirus	A large genus of plant viruses of the family POTYVIRIDAE which infect mainly plants of the Solanaceae. Transmission is primarily by aphids in a non-persistent manner. The type species is potato virus Y.	Potato Virus Y,Potato Virus Ys,Potyviruses,Virus Ys, Potato,Ys, Potato Virus
D018014	Gene Transfer Techniques	The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.	Gene Delivery Systems,Gene Transfer Technique,Transgenesis,Delivery System, Gene,Delivery Systems, Gene,Gene Delivery System,Technique, Gene Transfer,Techniques, Gene Transfer,Transfer Technique, Gene,Transfer Techniques, Gene
D018506	Gene Expression Regulation, Plant	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.	Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D030821	Plants, Genetically Modified	PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.	Genetically Modified Plants,Plants, Transgenic,Transgenic Plants,GMO Plants,Genetically Engineered Plants,Engineered Plant, Genetically,Engineered Plants, Genetically,GMO Plant,Genetically Engineered Plant,Genetically Modified Plant,Modified Plant, Genetically,Modified Plants, Genetically,Plant, GMO,Plant, Genetically Engineered,Plant, Genetically Modified,Plant, Transgenic,Plants, GMO,Plants, Genetically Engineered,Transgenic Plant