BIOMAT Database Logo BIOMAT Database Logo

Proteolysis of extracellular matrix by invadopodia facilitates human breast cancer cell invasion and is mediated by matrix metalloproteinases. 1998

T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Department of Pathology, Arkansas Cancer Research Center, University of Arkansas for Medical Sciences, Little Rock 72205-7199, USA. kellythomasj@exchange.uams.edu

Breast cancer cell lines vary in invasive behavior and one highly invasive cell line (MDA-MB-231) proteolytically degrades extracellular matrix with invadopodia (Thompson et al. 1992, J Cell Physiol, 150, 534-44; Chen et al 1994, Breast Cancer Res Treat, 31, 217-26). Invadopodial proteolysis of extracellular matrix is thought to be necessary for invasion; however, this has not been demonstrated directly. To obtain such evidence, normal (HBL-100) and malignant (MCF-7, MDA-MB-231) breast cells were evaluated for invadopodial proteolysis of extracellular matrix and invasive behavior. We report that invadopodial proteolysis of immobilized fibronectin is positively correlated with invasion of cells into type I collagen gels. Moreover, reducing the proteolytic activity of invadopodia with the metalloproteinase inhibitor, batimastat (BB-94), also decreases invasion indicating that breast cancer cell invasion is dependent upon proteolytically active invadopodia.

UI MeSH Term Description Entries
D008666 Metalloendopeptidases ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism. Metallo-Endoproteinases,Metalloendopeptidase
D009361 Neoplasm Invasiveness Ability of neoplasms to infiltrate and actively destroy surrounding tissue. Invasiveness, Neoplasm,Neoplasm Invasion,Invasion, Neoplasm
D009363 Neoplasm Proteins Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. Proteins, Neoplasm
D010649 Phenylalanine An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE. Endorphenyl,L-Phenylalanine,Phenylalanine, L-Isomer,L-Isomer Phenylalanine,Phenylalanine, L Isomer
D011554 Pseudopodia A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food. Axopodia,Filopodia,Lamellipodia,Lobopodia,Microspikes, Cell Surface,Reticulopodia,Pseudopodium,Cell Surface Microspike,Cell Surface Microspikes,Lamellipodias,Microspike, Cell Surface,Surface Microspike, Cell,Surface Microspikes, Cell
D001943 Breast Neoplasms Tumors or cancer of the human BREAST. Breast Cancer,Breast Tumors,Cancer of Breast,Breast Carcinoma,Cancer of the Breast,Human Mammary Carcinoma,Malignant Neoplasm of Breast,Malignant Tumor of Breast,Mammary Cancer,Mammary Carcinoma, Human,Mammary Neoplasm, Human,Mammary Neoplasms, Human,Neoplasms, Breast,Tumors, Breast,Breast Carcinomas,Breast Malignant Neoplasm,Breast Malignant Neoplasms,Breast Malignant Tumor,Breast Malignant Tumors,Breast Neoplasm,Breast Tumor,Cancer, Breast,Cancer, Mammary,Cancers, Mammary,Carcinoma, Breast,Carcinoma, Human Mammary,Carcinomas, Breast,Carcinomas, Human Mammary,Human Mammary Carcinomas,Human Mammary Neoplasm,Human Mammary Neoplasms,Mammary Cancers,Mammary Carcinomas, Human,Neoplasm, Breast,Neoplasm, Human Mammary,Neoplasms, Human Mammary,Tumor, Breast
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004791 Enzyme Inhibitors Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction. Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D005109 Extracellular Matrix A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere. Matrix, Extracellular,Extracellular Matrices,Matrices, Extracellular
D005260 Female Females

Related Publications

T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Liprin-α1 regulates breast cancer cell invasion by affecting cell motility, invadopodia and extracellular matrix degradation.
April 2011, Oncogene,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
EB1 Restricts Breast Cancer Cell Invadopodia Formation and Matrix Proteolysis via FAK.
February 2021, Cells,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Quantitative measurement of invadopodia-mediated extracellular matrix proteolysis in single and multicellular contexts.
August 2012, Journal of visualized experiments : JoVE,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Surface cholesterol-enriched domains specifically promote invasion of breast cancer cell lines by controlling invadopodia and extracellular matrix degradation.
July 2022, Cellular and molecular life sciences : CMLS,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Cancer cell metabolism regulates extracellular matrix degradation by invadopodia.
March 2013, European journal of cell biology,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Selective proteolysis by matrix metalloproteinases of photo-oxidised dermal extracellular matrix proteins.
February 2019, Cellular signalling,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Invadopodia promote proteolysis of a wide variety of extracellular matrix proteins.
February 1994, Journal of cellular physiology,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Msi1 promotes breast cancer metastasis by regulating invadopodia-mediated extracellular matrix degradation via the Timp3-Mmp9 pathway.
July 2021, Oncogene,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
[Matrix metalloproteinases and cancer cell invasion and metastasis].
July 1997, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
T Kelly, and Y Yan, and R L Osborne, and A B Athota, and T L Rozypal, and J C Colclasure, and W S Chu
Extracellular matrix metalloproteinases in tumor invasion and metastasis.
January 1991, Cancer treatment and research,
Copied contents to your clipboard!