-transparent.png){kind=logo}
Actin filaments partially cross-linked with ANP (N-(4-azido-2-nitrophenyl)-putrescine between Gln-41 and Cys-374 on adjacent monomers in the long-pitch helix were depolymerized and fractionated into pools of longitudinal cross-linked dimers (s(o)20,w = 5.55 +/- 0.22 S), trimers (s(o)20,w = 6.93 +/- 0.12 S), and higher-order oligomers. Competition binding experiments of myosin subfragment (S1) to cross-linked dimers in the presence of pyrenyl G-actin revealed about 2 orders of magnitude stronger binding of the first than that of the second S1 molecule to actin dimer. Under similar conditions the unpolymerized cross-linked actin species activated the MgATPase of S1 only severalfold compared to 70-fold activation by F-actin. The cross-linked dimers, trimers, and oligomers were polymerized into filaments by MgCl2 faster than un-cross-linked actin. In electron micrographs these filaments appeared sometimes shorter and had greater tendency to bend than un-cross-linked actin filaments. Small amounts of cross-linked actin dimers nucleated S1-induced polymerization of actin, but the polymerization by S1 was inhibited for pure populations of cross-linked dimers, trimers, and oligomers. The cross-linked dimers did not decrease the kinetic difference between the polymerization of actin by S1 isozymes S1(A1) and S1(A2). According to electron microscopy evidence, cross-linked actin oligomers polymerized by S1 yielded much shorter arrowhead structures than the un-cross-linked actin. These results indicate the importance of lateral actin-actin interaction for the activation of myosin ATPase and the polymerization of actin by S1.
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
December 1998,
Biochemistry,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
December 1998,
Biochemistry,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
January 1996,
Biochemistry,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
October 1985,
Biochemistry international,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
April 1992,
FEBS letters,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
January 1992,
Protein science : a publication of the Protein Society,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
October 2010,
Biochemistry,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
October 1987,
European journal of biochemistry,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
January 2003,
Free radical biology & medicine,
E Kim, and
M Phillips, and
G Hegyi, and
A Muhlrad, and
E Reisler
August 1984,
FEBS letters,
