Various parameter estimates were assessed at housing in calves that had been exposed to gastrointestinal nematodes during a first grazing season. The analysis involved 41 groups of first grazing season (FGS) calves on 15 different farms in Belgium and comprised groups that had received chemoprophylactic treatment and untreated controls. Serum pepsinogen levels gave the clearest division between chemoprophylactic-treated calf groups (all were <2.6 U tyr), and untreated calf groups in which sub-clinical (range: 2.0-4.1 U tyr) and clinical infections (range 3.7-6.3 U tyr) occurred. There was also a tight relationship between individual pepsinogen values and adult Ostertagia burdens obtained at slaughter. In chemoprophylactic-treated groups there was a significant negative relationship between mean serum pepsinogen levels at housing and the proportion of the grazing season covered by different chemoprophylactic systems. Although only limited data on crude adult Ostertagia antigen ELISA were available, a good relationship between optical densities and estimated exposure was also found. The parasitological parameters, faecal egg counts and pasture Ostertagia larval counts at housing, and weight gain per day, gave less clear divisions among the three categories (chemoprophylaxis, sub-clinical and clinical). Distinguishing how much exposure a calf group has experienced during a first grazing season could help in designing more appropriate control measures for the FGS calves in the next year, assuring good protection and at the same time allowing sufficient exposure for the development of acquired immunity to Ostertagia, and for this serum pepsinogen is recommended.