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Our work is focused on identifying micronuclei (MN) induced in whole blood lymphocyte cultures after treatment with the organic solvents toluene, benzene, and acetone. We used the micronucleus test as a cytogenetic biomarker for genotoxicity and treated whole blood cultures with different concentrations of these solvents (0.1 to 5 mM) and mixtures of them (toluene plus acetone, toluene plus benzene). Our results did not show a significant increase in the number of micronuclei in binucleated lymphocytes after 48 hr of in vitro treatment. The addition of an external metabolic factor (10% S9 mix for 2 hr) in blood cultures treated with the organic solvents or their mixtures did not cause induction of MN. These results indicate the lack of genotoxic activity of toluene, benzene, and acetone in vitro.
F Zarani, and
P Papazafiri, and
A Kappas
May 1988,
Mutation research,
F Zarani, and
P Papazafiri, and
A Kappas
December 2000,
Mutation research,
F Zarani, and
P Papazafiri, and
A Kappas
January 1995,
Environmental and molecular mutagenesis,
F Zarani, and
P Papazafiri, and
A Kappas
October 1987,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
F Zarani, and
P Papazafiri, and
A Kappas
September 1989,
International journal of radiation biology,
F Zarani, and
P Papazafiri, and
A Kappas
January 1993,
Environmental and molecular mutagenesis,
F Zarani, and
P Papazafiri, and
A Kappas
December 1990,
Toxicology letters,
F Zarani, and
P Papazafiri, and
A Kappas
July 1987,
Mutation research,
F Zarani, and
P Papazafiri, and
A Kappas
February 1994,
The American journal of clinical nutrition,
F Zarani, and
P Papazafiri, and
A Kappas
June 1999,
Chemosphere,
