Measles is an acute highly infectious viral disease. Although live attenuated vaccine is used throughout the world, outbreaks of disease still occur in many countries including Iran. In this cross-sectional study, by implementing a viral neutralization test and cell culture techniques, the seroprevalence of neutralizing anti-measles antibodies was assessed. Three hundred and fifty-four blood samples were collected and random-cluster classified from healthy subjects 6 months to 16 years old, residing in the town of Khodabandeh and its rural areas. Of the total subjects, 174 (49.2%) were girls and 180 (50.8%) were boys. From 354 subjects studied, 310 (87.6%) had neutralizing anti-measles antibody titer of 1:8 or higher and were considered to be immune and 44 (12.4%) had lower antibody titers. At the time of specimen collection, information with regards to age, sex, history of vaccination and place of residence were collected. Chi2 statistical test demonstrated a significant association between immune status and grouped age at the time of first vaccination (p < 0.009). The proportion test indicated significant differences in rate of seropositivity in paired age groups (3-8 vs. 9-11 and 9-11 vs. 12-64 months) (p < 0.02). The use of reliable techniques for assessing success of vaccination programs and performing seroepidemiological studies in order to organize national programs of control and eradication of measles are necessary.