Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats. 2010

R V Gonçalves, and J M S Mezêncio, and G P Benevides, and S L P Matta, and C A Neves, and M M Sarandy, and E F Vilela
Departamento de Biologia Geral, Universidade Federal de Viçosa, Edifício Shotaro Shymoia, Avenida PH Rolfs, s/n, 35570-000 Viçosa, MG, Brasil. reggysvilela@yahoo.com.br

This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals' backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm(2)); group 2 (L30): GaAlAs laser (30 J/cm(2)); group 3 (L60): GaAlAs laser (60 J/cm(2)); group 4 (D): Dersani ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 + or - 7.10%), which differed from control (29.86 + or - 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 + or - 2.23%) and group 2 (L30; 2.87 + or - 2.40%) differed significantly from control (1.77 + or - 2.97%) on the 20th day of the experiment.

UI MeSH Term Description Entries
D008297 Male Males
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012867 Skin The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D014945 Wound Healing Restoration of integrity to traumatized tissue. Healing, Wound,Healings, Wound,Wound Healings
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D054023 Lasers, Semiconductor Lasers with a semiconductor diode as the active medium. Diode lasers transform electric energy to light using the same principle as a light-emitting diode (LED), but with internal reflection capability, thus forming a resonator where a stimulated light can reflect back and forth, allowing only a certain wavelength to be emitted. The emission of a given device is determined by the active compound used (e.g., gallium arsenide crystals doped with aluminum or indium). Typical wavelengths are 810, 1,060 and 1,300 nm. (From UMDNS, 2005) Diode Lasers,Gallium Aluminum Arsenide Lasers,Gallium Arsenide Lasers,GaAlAs Lasers,GaAs Lasers,Lasers, GaAlAs,Lasers, GaAs,Lasers, Gallium Aluminum Arsenide,Lasers, Gallium Arsenide,Lasers, Quantum Cascade,Quantum Cascade Lasers,Semiconductor Diode Lasers,Arsenide Laser, Gallium,Arsenide Lasers, Gallium,Diode Laser,Diode Laser, Semiconductor,Diode Lasers, Semiconductor,GaAlAs Laser,GaAs Laser,Gallium Arsenide Laser,Laser, Diode,Laser, GaAlAs,Laser, GaAs,Laser, Gallium Arsenide,Laser, Quantum Cascade,Laser, Semiconductor,Laser, Semiconductor Diode,Lasers, Diode,Lasers, Semiconductor Diode,Quantum Cascade Laser,Semiconductor Diode Laser,Semiconductor Laser,Semiconductor Lasers
D024042 Collagen Type I The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains. Type 1 Collagen,Type I Collagen,Collagen, Type 1,Collagen, Type I
D024061 Collagen Type III A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics. Collagen Type III, alpha1 Chain,Collagen alpha1(III),Procollagen Type III,Type III Collagen,Type III Procollagen,Collagen, Type III
D028022 Low-Level Light Therapy Treatment using irradiation with light of low power intensity so that the effects are a response to the light and not due to heat. A variety of light sources, especially low-power lasers are used. LLLT,Laser Biostimulation,Laser Irradiation, Low-Power,Laser Therapy, Low-Level,Photobiomodulation,Laser Phototherapy,Laser Therapy, Low-Power,Low-Level Laser Therapy,Low-Power Laser Irradiation,Low-Power Laser Therapy,Photobiomodulation Therapy,Biostimulation, Laser,Irradiation, Low-Power Laser,Laser Irradiation, Low Power,Laser Therapies, Low-Level,Laser Therapies, Low-Power,Laser Therapy, Low Level,Laser Therapy, Low Power,Light Therapies, Low-Level,Light Therapy, Low-Level,Low Level Laser Therapy,Low Level Light Therapy,Low Power Laser Irradiation,Low Power Laser Therapy,Low-Level Laser Therapies,Low-Level Light Therapies,Low-Power Laser Therapies,Photobiomodulation Therapies,Photobiomodulations,Phototherapy, Laser,Therapies, Low-Level Light,Therapies, Photobiomodulation,Therapy, Low-Level Light,Therapy, Photobiomodulation

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