Comparative study of the effects of gallium-aluminum-arsenide laser photobiomodulation and healing oil on skin wounds in wistar rats: a histomorphometric study. 2010

Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
Laboratory of Structural Biology, Department of General Biology, Federal University of Viçosa, MG, Brazil. reggysvilela@yahoo.com.br

OBJECTIVE The present study compared the effects of gallium-aluminum-arsenide diode laser and healing oil on fibroblasts, blood vessels, and collagen maturation of skin wounds in Wistar rats. METHODS Twenty-four male rats weighing 325 ± 27 g were used. Five wounds, 12 mm in diameter, were made on the animals' backs. The rats were randomly divided into four groups with six animals in each group. METHODS saline solution; L30 group: 30 J/cm(2) laser; L60 group: 60 J/cm(2) laser; Oil group: healing oil. Histomorphometric analysis was performed on the scar tissue removed from the different wounds every 4 d for 20 d. RESULTS On day 4, there were significantly more fibroblasts in the wounds treated with the laser and the healing oil compared to the controls. On day 8, there were significantly more fibroblasts in the oil group compared to the L30 and L60 groups. On the same day, the quantity of vessels was significantly greater in the L60 group compared to the other groups. On day 16, there was a significant increase in the number of blood vessels in the wounds treated with the 60 J/cm(2) laser compared to the other groups. Analysis of the collagen maturation index throughout the experiment showed significantly higher values in the L60 group compared to the other groups at all time points. CONCLUSIONS The healing oil exerted a greater effect on fibroblast proliferation, whereas the 60 J/cm(2) laser was more effective in stimulating angiogenesis and scar-tissue maturation.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297 Male Males
D010938 Plant Oils Oils derived from plants or plant products. Oils, Plant,Oils, Vegetable,Plant Oil,Vegetable Oil,Vegetable Oils,Oil, Plant,Oil, Vegetable
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D012016 Reference Values The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality. Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D000287 Administration, Topical The application of drug preparations to the surfaces of the body, especially the skin (ADMINISTRATION, CUTANEOUS) or mucous membranes. This method of treatment is used to avoid systemic side effects when high doses are required at a localized area or as an alternative systemic administration route, to avoid hepatic processing for example. Drug Administration, Topical,Administration, Topical Drug,Topical Administration,Topical Drug Administration,Administrations, Topical,Administrations, Topical Drug,Drug Administrations, Topical,Topical Administrations,Topical Drug Administrations
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
April 2010, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
December 2009, Photomedicine and laser surgery,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
August 2010, Photomedicine and laser surgery,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
January 1997, Lasers in surgery and medicine,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
February 2010, Synapse (New York, N.Y.),
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
August 2008, Photomedicine and laser surgery,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
April 2008, Photomedicine and laser surgery,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
January 2017, Journal of Indian Society of Periodontology,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
February 2010, Photomedicine and laser surgery,
Reggiani V Gonçalves, and Rômulo D Novaes, and Sérgio L P Matta, and Gustavo P Benevides, and Fanciane R Faria, and Marcus V M Pinto
January 1991, Scandinavian journal of plastic and reconstructive surgery and hand surgery,
Copied contents to your clipboard!