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A simplified method of processing of fine needle aspirates for paraffin miniblocks suitable for both morphologic and immunocytochemical evaluation is described. Aspirates were fixed in ethanol at 4 degrees C, dehydrated in acetone and xylene and embedded in paraffin (58 degrees C). All steps were carried out in a single Eppendorf centrifuge tube; the total process took less than four hours. Deparaffinized sections were stained using the alkaline phosphatase-antialkaline phosphatase technique with monoclonal and conventional antibodies helpful in the differential cytologic diagnosis of alcohol-fixed aspiration biopsy specimens. Antibodies to keratin, vimentin, desmin, neurofilaments, glial fibrillary acidic protein, leukocyte-common antigen, synaptophysin and immunoglobulin kappa and lambda light chains reacted positively on the miniblock material. Since the paraffin miniblocks combine the histologic pattern of the tumor with the differentiation-specific information provided by immunocytochemistry, their use can improve the accuracy of tumor typing in aspirates.
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
January 1990,
Acta cytologica,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
August 1998,
The British journal of oral & maxillofacial surgery,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
February 2013,
Cytopathology : official journal of the British Society for Clinical Cytology,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
March 1991,
The Australian and New Zealand journal of surgery,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
January 1989,
Acta cytologica,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
December 2014,
Cancer cytopathology,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
July 2012,
Journal of cytology,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
June 2013,
American journal of clinical pathology,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
January 1992,
Acta cytologica,
W M Domagala, and
M Markiewski, and
T Tuziak, and
A Kram, and
K Weber, and
M Osborn
January 1996,
Acta cytologica,
