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Epithelial cells from the toad urinary bladder have been grown in continuous culture. Many of the cells resemble the granular cell type of the urinary bladder. They form an epithelium with typical tight junctions and gap junctions. The transport properties of two cell lines have been examined. When cells of the line designated TB-M or of line TB-6c are grown on collagen-coated nucleopore filters, epithelia are formed that have transepithelial potential differences of 40 and 20 mV, resistances of 5000 and 10,000 omega-cm2, and short-circuit currents (ISC) of 8.5 and 2.5 muA/cm2, respectively. Net mucosa to serosa sodium transport accounts for all of ISC in line TB-M and for 70% of ISC in line TB-6c. Vasopressin, which stimulates adenylate cylase and ISC in the intact bladder, has no effect on the cells in culture. Cyclic AMP stimulates ISC and lowers resistance in both lines. Aldosterone stimulates ISC in both lines. This is accompanied by a fall in resistance in line TB-M and no change in resistance in line TB-6c. Amiloride inhibits ISC in TB-M cells under basal conditions and after stimulation by aldosterone. In line TB-6c amiloride has no effect under basal conditions but lowers ISC of aldosterone-treated cells to the basal level. Thus, the cells have retained the ability to form oriented, high-resistance epithelial membranes that manifest hormone-sensitive transepithelial sodium transport.
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
January 1975,
The Journal of membrane biology,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
January 1978,
The Journal of membrane biology,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
September 1978,
Proceedings of the National Academy of Sciences of the United States of America,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
March 1986,
Biochimica et biophysica acta,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
April 1977,
The Journal of membrane biology,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
June 1960,
Experimental cell research,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
September 1974,
The American journal of physiology,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
October 1971,
The American journal of physiology,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
August 1986,
The Journal of pharmacology and experimental therapeutics,
J S Handler, and
R E Steele, and
M K Sahib, and
J B Wade, and
A S Preston, and
N L Lawson, and
J P Johnson
March 1978,
The American journal of physiology,
