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Data are described on identification, isolation and purification of restricting endonuclease Sau 6782 as well as on estimation of the enzyme recognition site. Conditions were developed for growing of Staphylococcus aureus 6782 strain, which enabled to produce a maximal yield of the restricting activity containing minimal level of nucleases. The procedure for isolation and purification of restrictase Sau 6782 involved affinity chromatography on Blue Sepharose and cation exchange chromatography on phosphocellulose PII. The enzyme preparation obtained was free from impurities of unspecific nucleases. The yield of the Sau 6782 restrictase constituted 1,000 un from 1 g of the culture cells. Restrictase Sau 6782 recognized the nucleotide sequence 5'...GATC...3' and was the isoshizomere of the Sau 3A enzyme.
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
January 1992,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
January 1990,
Voprosy meditsinskoi khimii,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
February 1991,
Biokhimiia (Moscow, Russia),
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
December 1984,
Nucleic acids research,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
February 1994,
Proceedings of the National Academy of Sciences of the United States of America,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
March 1987,
Bioorganicheskaia khimiia,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
January 1987,
Gene amplification and analysis,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
April 1989,
Nucleic acids research,
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
January 1993,
Methods in molecular biology (Clifton, N.J.),
E E Arutiuniunian, and
I M Gruber, and
V M Poliachenko, and
L I Kvachadze, and
I A Andriashvili
November 1974,
Journal of molecular biology,
