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Monoclonal antibodies to human acrosin were required for studies of immunological interference with fertilization. Since human acrosin was not available in adequate amounts, monoclonal antibodies have been raised in mice against purified bovine acrosin and screened for cross-reaction with human sperm cells. Two of these antibodies are described, B4F6 and C2E5. Data from enzyme-linked immunosorbent assays, immunoblots, immunoprecipitation, and indirect immunofluorescence on sperm cells indicate that B4F6 binds only to bovine acrosin, and that C2E5 binds both to bovine and to human acrosin at a conformationally determined epitope. The antibodies do not inhibit the hydrolysis of benzoylarginine ethyl ester by acrosin, but C2E5 did inhibit the dissolution of the hamster zona pellucida by purified human acrosin. The antibodies have also been used for affinity purification of acrosin and proacrosin.
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
January 1982,
Canadian journal of biochemistry,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
May 1993,
Biochemical Society transactions,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
October 1987,
Hybridoma,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
January 1990,
Problemy endokrinologii,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
October 1987,
Collagen and related research,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
January 1985,
Current eye research,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
January 1986,
Animal genetics,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
July 1984,
Veterinary immunology and immunopathology,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
December 1987,
Gamete research,
J S Elce, and
E J Graham, and
G Zboril, and
L Leyton, and
E Perez, and
H B Croxatto, and
A de Ioannes
January 1988,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
