Biomaterial Database

Muscarinic cholinergic receptors on intact human lymphocytes. Properties and subclass characterization. 1987

B Bering, and H W Moises, and W E Müller

Psychopharmacological Laboratory, Central Institute of Mental Health, Mannheim, F.R.G.

Saturable specific binding of tritiated N-methyl-scopolamine (3H-NMS) can be demonstrated on intact circulating human lymphocytes, with an average KD of 7 nmol/liter and an average density of about 15 fmol/10(6) cells. Specific 3H-NMS binding can be inhibited by cholinergic antagonists and agonists, is highly stereospecific for the enantiomers of the cholinergic antagonist quinuclidinyl benzilate (QNB), and is modulated by the stable guanosine triphosphate (GTP) analog GppNHp in a fashion similar to the specific binding of the same radioligand to rat heart membranes. The results indicate the presence of muscarinic cholinergic receptors on intact human lymphocytes, with a predominance of the M2 subtype. As central muscarinic cholinergic receptors have been reported to play an important role in the pathogenesis of affective disorders, the lymphocyte might represent a suitable model for the study of muscarinic receptor functions in humans.

UI	MeSH Term	Description	Entries
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D011976	Receptors, Muscarinic	One of the two major classes of cholinergic receptors. Muscarinic receptors were originally defined by their preference for MUSCARINE over NICOTINE. There are several subtypes (usually M1, M2, M3....) that are characterized by their cellular actions, pharmacology, and molecular biology.	Muscarinic Acetylcholine Receptors,Muscarinic Receptors,Muscarinic Acetylcholine Receptor,Muscarinic Receptor,Acetylcholine Receptor, Muscarinic,Acetylcholine Receptors, Muscarinic,Receptor, Muscarinic,Receptor, Muscarinic Acetylcholine,Receptors, Muscarinic Acetylcholine
D003866	Depressive Disorder	An affective disorder manifested by either a dysphoric mood or loss of interest or pleasure in usual activities. The mood disturbance is prominent and relatively persistent.	Depression, Endogenous,Depression, Neurotic,Depression, Unipolar,Depressive Syndrome,Melancholia,Neurosis, Depressive,Unipolar Depression,Depressions, Endogenous,Depressions, Neurotic,Depressions, Unipolar,Depressive Disorders,Depressive Neuroses,Depressive Neurosis,Depressive Syndromes,Disorder, Depressive,Disorders, Depressive,Endogenous Depression,Endogenous Depressions,Melancholias,Neuroses, Depressive,Neurotic Depression,Neurotic Depressions,Syndrome, Depressive,Syndromes, Depressive,Unipolar Depressions
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001667	Binding, Competitive	The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.	Competitive Binding
D012602	Scopolamine Derivatives	Analogs or derivatives of scopolamine.	Scopolamines,Derivatives, Scopolamine
D019832	N-Methylscopolamine	A muscarinic antagonist used to study binding characteristics of muscarinic cholinergic receptors.	DD-234,Hyoscine Methiodide,Hyoscine Methobromide,Methscopolamine,Methylscopolamine Nitrate,Methylscopolammonium Methylsulfate,N-Methylscine Methylsulfate,N-Methylscopolamine Bromide,N-Methylscopolamine Iodide,N-Methylscopolamine Methylchloride,N-Methylscopolamine Methylsulfate,N-Methylscopolamine Nitrate,Scopolamine Methylbromide,Skopyl,Ulix,Bromide, N-Methylscopolamine,DD 234,DD234,Iodide, N-Methylscopolamine,Methiodide, Hyoscine,Methobromide, Hyoscine,Methylbromide, Scopolamine,Methylchloride, N-Methylscopolamine,Methylsulfate, Methylscopolammonium,Methylsulfate, N-Methylscine,Methylsulfate, N-Methylscopolamine,N Methylscine Methylsulfate,N Methylscopolamine,N Methylscopolamine Bromide,N Methylscopolamine Iodide,N Methylscopolamine Methylchloride,N Methylscopolamine Methylsulfate,N Methylscopolamine Nitrate,Nitrate, Methylscopolamine,Nitrate, N-Methylscopolamine