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The N-glycosidic carbohydrate chains of Panulirus interruptus hemocyanin, consisting of only mannose and N-acetylglucosamine residues, were liberated by hydrazinolysis of a pronase digest of the purified glycoprotein. The carbohydrate chains were fractionated by high-voltage paper electrophoresis, yielding mainly neutral oligosaccharide-alditols. These were further separated by high-performance liquid chromatography and characterized by 500-MHz 1H-NMR spectroscopy as (formula; see text).
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