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A multifunctional protein possessing glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase activities in de novo purine biosynthesis. 1985

S C Daubner, and J L Schrimsher, and F J Schendel, and M Young, and S Henikoff, and D Patterson, and J Stubbe, and S J Benkovic

Three activities on the pathway of purine biosynthesis de novo in chicken liver, namely, glycinamide ribonucleotide synthetase, glycinamide ribonucleotide transformylase, and aminoimidazole ribonucleotide synthetase, have been found to reside on the same polypeptide chain. Three diverse purification schemes, utilizing three different affinity resins, give rise to the same protein since the final material has identical specific activities for all three enzymatic reactions and a molecular weight on sodium dodecyl sulfate gels of about 110 000. A single antibody preparation precipitates all three activities and binds to the multifunctional protein obtained by two methods in Western blots. Partial chymotryptic digestion of the purified protein gives rise to two fragments, one possessing glycinamide ribonucleotide synthetase activity and the other containing glycinamide ribonucleotide transformylase activity.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008025 Ligases A class of enzymes that catalyze the formation of a bond between two substrate molecules, coupled with the hydrolysis of a pyrophosphate bond in ATP or a similar energy donor. (Dorland, 28th ed) EC 6. Ligase,Synthetases,Synthetase
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D009097 Multienzyme Complexes Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES. Complexes, Multienzyme
D011687 Purines A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000217 Acyltransferases Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3. Acyltransferase
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051026 Phosphoribosylglycinamide Formyltransferase An enzyme that catalyzes the transfer of a formyl group from N10-formyltetrahydrofolate to N1-(5-phospho-D-ribosyl)glycinamide to yield N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and tetrahydrofolate. It plays a role in the de novo purine biosynthetic pathway. 2-Amino-N-Ribosylacetamide 5'-Phosphate Transformylase,5'-Phosphoribosylglycinamide Transformylase,GAR Formyltransferase,GAR Transformylase,GARTFase,Glycinamide Ribonucleotide Transformylase,Glycinamideribotide Transformylase,2 Amino N Ribosylacetamide 5' Phosphate Transformylase,5' Phosphoribosylglycinamide Transformylase,5'-Phosphate Transformylase, 2-Amino-N-Ribosylacetamide,Formyltransferase, GAR,Formyltransferase, Phosphoribosylglycinamide,Ribonucleotide Transformylase, Glycinamide,Transformylase, 2-Amino-N-Ribosylacetamide 5'-Phosphate,Transformylase, 5'-Phosphoribosylglycinamide,Transformylase, GAR,Transformylase, Glycinamide Ribonucleotide,Transformylase, Glycinamideribotide
D019731 Carbon-Nitrogen Ligases Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3. Carbon Nitrogen Ligases,Ligases, Carbon-Nitrogen

Related Publications

S C Daubner, and J L Schrimsher, and F J Schendel, and M Young, and S Henikoff, and D Patterson, and J Stubbe, and S J Benkovic
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On the cofactor specificity of glycinamide ribonucleotide and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase from chicken liver.
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Assignment of a third purine biosynthetic gene (glycinamide ribonucleotide transformylase) to human chromosome 21.
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De novo purine ribonucleotide biosynthesis in adult Angiostrongylus cantonensis (Nematoda: Metastrongyloidea).
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