Biomaterial Database

beta-Nerve growth factor (beta NGF) receptors on glial cells. Cell-cell interaction between neurones and Schwann cells in cultures of chick sensory ganglia. 1983

A Zimmermann, and A Sutter

Receptors for beta-nerve growth factor (beta NGF), so far regarded as specific cell surface markers of certain peripheral neurones, were found to be expressed on cultured non-neuronal cells of chick embryo dorsal root ganglia (drg) (Kd beta NGF = 2 X 10(-9) M). Autoradiography revealed that binding of [125I] beta NGF was restricted to a subpopulation of the non-neuronal drg cells. Cultured embryonic skin fibroblasts, liver cells, gut cells, muscle fibroblasts, myoblasts, and myotubes, as well as macrophages and the cell lines 3T3, 3T3SV40, BHK, BHK Py, PCC3 and ND1, did not express receptors for beta NGF. Non-neuronal drg cells obtained by a procedure designed for the preparation of pure Schwann cells, as well as RN6 Schwannoma cells, were beta NGF receptor positive. The beta NGF receptor-positive non-neuronal drg cells displayed behaviour typical of Schwann cells in their interaction with drg neurones in single cell, as well as explant cultures. Three stages of neurone-Schwann cell interaction were discernible: (1) association--neurites preferentially grew over beta NGF receptor-positive non-neuronal cells; (2) cell division/alignment--beta NGF receptor-positive non-neuronal cells were induced to proliferate and aligned and elongated along neurites; (3) ensheathment--the outline of beta NGF receptor-positive non-neuronal cells and neurites merged. In drg cell cultures prepared from embryonic stages E6-E10, 25-40% of the non-neuronal cells were beta NGF receptor-positive. Later in development, from E12 onward, less than or equal to 1% of the cultured non-neuronal cells expressed beta NGF receptors.

UI	MeSH Term	Description	Entries
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D002450	Cell Communication	Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.	Cell Interaction,Cell-to-Cell Interaction,Cell Communications,Cell Interactions,Cell to Cell Interaction,Cell-to-Cell Interactions,Communication, Cell,Communications, Cell,Interaction, Cell,Interaction, Cell-to-Cell,Interactions, Cell,Interactions, Cell-to-Cell
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D005727	Ganglia, Spinal	Sensory ganglia located on the dorsal spinal roots within the vertebral column. The spinal ganglion cells are pseudounipolar. The single primary branch bifurcates sending a peripheral process to carry sensory information from the periphery and a central branch which relays that information to the spinal cord or brain.	Dorsal Root Ganglia,Spinal Ganglia,Dorsal Root Ganglion,Ganglion, Spinal,Ganglia, Dorsal Root,Ganglion, Dorsal Root,Spinal Ganglion
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012583	Schwann Cells	Neuroglial cells of the peripheral nervous system which form the insulating myelin sheaths of peripheral axons.	Schwann Cell,Cell, Schwann,Cells, Schwann
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D017475	Receptors, Nerve Growth Factor	Cell surface receptors that bind NERVE GROWTH FACTOR; (NGF) and a NGF-related family of neurotrophic factors that includes neurotrophins, BRAIN-DERIVED NEUROTROPHIC FACTOR and CILIARY NEUROTROPHIC FACTOR.	NGF Receptors,Nerve Growth Factor Receptors,Neurotrophic Factor Receptor,Neurotrophin Receptor,Receptors, NGF,Receptors, Neurotrophin,Neurotrophin Receptors,Receptors, Neurotrophic Factor,Neurotrophic Factor Receptors,Receptor, Neurotrophic Factor,Receptor, Neurotrophin