-transparent.png){kind=logo}
Human leukemic cells which bear the common acute lymphoblastic leukemia antigen can be lysed with a murine monoclonal antibody (J-5) in the presence of rabbit complement. Conditions have been defined for eliminating 51Cr-labeled common acute lymphoblastic leukemia antigen-positive NALM-1 cells or cryopreserved leukemic lymphoblasts from a 100-fold excess of human bone marrow. Optimal lysis is obtained with treatment for a total of 90 min. Three treatments for 30 min are more effective than two treatments for 45 min or one treatment for 90 min. Separation of marrow on a Ficoll:diatrizoate gradient does not permit more effective elimination of leukemic cells. Tumor cell lysis is inhibited by high concentrations of common acute lymphoblastic leukemia antigen-positive cells (2 X 10(7)/ml) and by high concentrations of bone marrow (10(8)/ml). Under optimal conditions, greater than 99% of 51Cr-labeled leukemic lymphoblasts can be eliminated from a 100-fold excess of human marrow. Selective removal of leukemic cells from human bone marrow in vitro should facilitate trials of autologous marrow transplantation.
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
September 1981,
Cancer research,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
January 1990,
Progress in clinical and biological research,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
January 1990,
Progress in clinical and biological research,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
August 1991,
Chinese medical journal,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
November 1983,
Lancet (London, England),
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
April 1985,
Blood,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
August 1984,
Journal of biological response modifiers,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
February 1983,
Transplantation,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
January 1993,
Anticancer research,
R C Bast, and
J Ritz, and
J M Lipton, and
M Feeney, and
S E Sallan, and
D G Nathan, and
S F Schlossman
February 1982,
British journal of haematology,
