-transparent.png){kind=logo}
An effective method for protoplast fusion in S. griseus producing grisin was developed. The method requires the use of polyethylene glycol with a molecular weight of 1000. It was demonstrated that protoplasts formed most effectively in this organism, when the mycelium of the streptomycete previously treated with ultrasound in the process of its growth was used for the treatment with lysozyme. The efficacy of protoplast regeneration in the strains with the use of the modified hypertonic medium R2MD was 25-75 per cent. The possibility of using the protoplast fusion method for constructing phage resistant strains producing kormogrisin was shown.
Related Publications
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
March 1988,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
June 1977,
The Journal of antibiotics,
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
January 1990,
Chinese journal of biotechnology,
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
June 1979,
Antibiotiki,
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
January 1996,
Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993),
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
June 1993,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
January 1985,
The Journal of antibiotics,
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
January 1994,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
January 1986,
Hindustan antibiotics bulletin,
V V Kuklin, and
L K Emel'ianova, and
V G Zhdanov, and
L S Iustratova
January 1982,
Zeitschrift fur allgemeine Mikrobiologie,
