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Heartwater was diagnosed in sheep at the Umm Banein Livestock Research Station, Blue Nile Province, Sudan. Cowdria ruminantium was isolated from sheep by subinoculation of blood into a goat. Adult Amblyomma lepidum ticks fed as nymphs on this goat transmitted heartwater to a Friesian (Bos taurus) calf. Brain stabilates from this calf, either snap-frozen into liquid nitrogen or frozen slowly in a deepfreezer at -70 degrees C, with DMSO as cryoprotectant, were infective to cattle when inoculated subcutaneously.
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