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Ten single-copy DNA probes derived from a human chromosome 3-specific genomic library were mapped by in situ hybridization to subregions of this chromosome. Seven sequences were assigned to subregions of 3q and two sequences were assigned to subregions of 3p. One single-copy DNA probe was assigned to the centromeric region of chromosome 3 by Southern blot analysis of DNA isolated from a somatic cell hybrid containing centromeric sequences of this chromosome. These DNA clones mapped by in situ hybridization can provide useful landmarks for mapping various disease loci on chromosome 3. They may also be useful for the generation of physical and genetic maps.
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