-transparent.png){kind=logo}
The 32P-postlabeling method was adapted for the detection of DNA adducts formed by methylenediphenyl-4,4'-diisocyanate (MDI). Incubation of the 3'-phosphates of the deoxyribosides of cytosine (C), adenine (A), guanine (G) and thymine (T) with MDI in Tris buffer resulted in the formation of 5, 7, 8, and 2 reaction products, respectively. Incubation of DNA with MDI resulted in detectable levels of 5, 2, and 1 adducts attributable to C, A, and G. Analysis of DNA isolated from the epidermis of rats treated dermally with 9 mg MDI showed an adduct pattern similar to the one seen in the in vitro DNA incubation. A total adduct level of 7 per 10(8) nucleotides was measured, the limit of detection was 2 adducts per 10(10) nucleotides. The data indicate that a minute fraction of MDI can reach DNA in vivo in a chemically reactive form. In comparison with the genotoxic skin carcinogen 7,12-dimethylbenz[a]anthracene on the other hand, the DNA-binding potency of MDI was more than 1000-fold lower.
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
December 2009,
Chemical research in toxicology,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
January 1999,
IARC monographs on the evaluation of carcinogenic risks to humans,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
May 2010,
Environmental and molecular mutagenesis,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
May 1996,
Carcinogenesis,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
July 2004,
Archives of toxicology,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
January 1989,
Chemical research in toxicology,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
July 2011,
Mutation research,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
July 1997,
Toxicology letters,
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
January 2020,
Methods in molecular biology (Clifton, N.J.),
E H Vock, and
S Cantoreggi, and
R C Gupta, and
W K Lutz
January 2005,
Methods in molecular biology (Clifton, N.J.),
